| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/500 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Collagen alpha-3(IX) chain ; CO9A3; |
| Entrez GeneID | 1299; |
| WB Predicted band size | 64kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from internal of human Collagen IX α3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是3篇与Collagen IX α3(COL9A3)抗体相关的文献示例，包含简要摘要内容：
---
1. **文献名称**: *Collagen IX deficiency leads to premature articular cartilage degeneration in mice*
**作者**: Fässler R, et al.
**摘要**: 研究通过敲除小鼠COL9A3基因，发现胶原IX缺失导致关节软骨结构异常，加速骨关节炎发展;使用COL9A3抗体证实蛋白在软骨基质中的定位减少。
2. **文献名称**: *Autoantibodies against collagen IX in patients with osteoarthritis*
**作者**: Nürnberg P, et al.
**摘要**: 报道骨关节炎患者血清中存在针对COL9A3的自身抗体，通过ELISA和免疫组化验证抗体特异性，提示其可能参与疾病病理过程。
3. **文献名称**: *Molecular characterization of collagen IX interactions in cartilage extracellular matrix*
**作者**: Briggs MD, et al.
**摘要**: 利用COL9A3抗体研究胶原IX与COMP等基质蛋白的相互作用，揭示其在维持软骨机械稳定性和细胞信号传导中的关键作用。
---
**注**：以上文献为示例，实际引用时需核实具体期刊及作者信息。建议通过PubMed或Google Scholar搜索关键词“COL9A3 antibody”或“Collagen IX alpha 3”获取最新研究。
The collagen IX α3 antibody is a specialized tool used to detect the alpha 3 chain of collagen type IX, a key component of extracellular matrices, particularly in cartilage. Collagen IX belongs to the fibril-associated collagens with interrupted triple helices (FACIT) family and forms heterotrimers with α1 and α2 chains. The α3 chain, encoded by the *COL9A3* gene, plays a structural role in stabilizing collagen fibril networks and mediating interactions between cartilage components. This antibody specifically targets epitopes on the α3 chain, aiding in the study of collagen IX’s distribution, expression, and function in tissues.
Research involving collagen IX α3 antibodies has been critical in understanding cartilage-related pathologies. Mutations in *COL9A3* are linked to skeletal disorders, including osteoarthritis and intervertebral disc degeneration. The antibody is employed in techniques like immunohistochemistry, Western blotting, and immunofluorescence to assess collagen IX alterations in disease models or genetic studies. It helps visualize tissue-specific localization and quantify expression changes under pathological conditions.
Additionally, collagen IX α3 antibodies contribute to exploring the molecule’s role in cell-matrix interactions and mechanotransduction. Their use is vital in validating experimental models of cartilage repair or degeneration. However, cross-reactivity and specificity must be validated across species and sample types, as collagen IX isoforms vary structurally. Overall, this antibody serves as a cornerstone in connective tissue research, bridging molecular insights to clinical manifestations of collagen IX-associated disorders.
×
