| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/500 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CO5A3; collagen alpha 3; |
| Entrez GeneID | 50509; |
| WB Predicted band size | 170kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from internal of human Collagen V α3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Collagen V α3抗体的3篇参考文献示例(注：文献信息为模拟，建议通过学术数据库核实)：
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1. **文献名称**：*Autoantibodies to Collagen V α3 Chain in Chronic Lung Allograft Dysfunction*
**作者**：Moghaddam, S.J. et al.
**摘要**：研究探讨了肺移植患者中抗Collagen V α3(COL5A3)自身抗体的存在与慢性排斥反应(如闭塞性细支气管炎)的关联。通过ELISA检测发现，患者血清中高水平的抗COL5A3抗体与移植物功能恶化显著相关，提示其可能作为预后生物标志物。
2. **文献名称**：*Collagen V α3 as a Target in Systemic Sclerosis Pathogenesis*
**作者**：Farina, G. et al.
**摘要**：分析了系统性硬化症(硬皮病)患者中抗COL5A3抗体的作用，发现这些抗体与皮肤和肺纤维化程度相关。研究通过免疫印迹法证实抗体与胶原V的α3链特异性结合，为自身免疫性纤维化的机制提供了新见解。
3. **文献名称**：*Collagen V α3 Expression in Tumor Stroma Promotes Breast Cancer Metastasis*
**作者**：Adair-Kirk, T.L. et al.
**摘要**：利用抗COL5A3抗体进行免疫组化分析，揭示胶原V α3在乳腺癌微环境中的高表达与肿瘤侵袭性增强相关。研究提示靶向COL5A3可能抑制转移途径。
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**提示**：以上文献信息为示例，实际引用时请通过PubMed或Google Scholar核对作者、年份及摘要准确性。如需更多近期研究，可搜索关键词“COL5A3 antibody”或“Collagen V alpha3 autoantibody”。
Collagen V α3 (COL5A3) is a minor fibrillar collagen component encoded by the *COL5A3* gene. It plays a critical role in regulating the assembly and stability of heterotypic collagen fibrils, primarily forming heterotrimers with α1 and α2 chains of collagen V. These fibrils serve as structural scaffolds in tissues such as skin, tendons, and blood vessels, contributing to extracellular matrix (ECM) organization. COL5A3 is also involved in cell-matrix interactions, influencing tissue repair, fibrosis, and cellular signaling pathways.
Antibodies targeting COL5A3 are essential tools for studying its expression, localization, and function in both physiological and pathological contexts. Research has linked COL5A3 dysregulation to fibrotic disorders (e.g., systemic sclerosis, idiopathic pulmonary fibrosis), cancer progression (e.g., tumor stroma remodeling, metastasis), and connective tissue abnormalities. In autoimmune diseases like rheumatoid arthritis, COL5A3 antibodies may detect altered collagen processing or immune targeting.
Clinically, COL5A3 antibodies are used in immunohistochemistry, Western blotting, or ELISA to assess tissue-specific expression patterns or biomarker potential. Their development also supports therapeutic exploration, such as blocking aberrant collagen deposition in fibrosis. However, challenges remain in distinguishing COL5A3 from other collagen isoforms due to structural homology. Ongoing studies aim to refine antibody specificity and clarify COL5A3’s mechanistic roles in disease pathways.
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