| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CDK5 regulatory subunit-associated protein 1-like 1; CDKAL; |
| Entrez GeneID | 54901; |
| WB Predicted band size | 65kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | Synthesized peptide derived from N-terminal of human CDKAL. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于CDKAL抗体的3篇参考文献及其简要摘要：
---
1. **文献名称**: *CDKAL1 regulates insulin secretion by modulating mitochondrial function in pancreatic β-cells*
**作者**: Ohara-Imaizumi M, et al.
**期刊/年份**: *Diabetologia* (2016)
**摘要**: 该研究通过免疫荧光和Western blot技术，利用CDKAL1特异性抗体揭示其在胰岛β细胞线粒体中的定位。结果表明，CDKAL1缺失导致线粒体功能异常，进而损害葡萄糖刺激的胰岛素分泌，提示其与2型糖尿病发病机制相关。
---
2. **文献名称**: *CDKAL1 variants modulate proinsulin homeostasis in humans*
**作者**: Yamauchi T, et al.
**期刊/年份**: *Journal of Clinical Investigation* (2010)
**摘要**: 通过ELISA和免疫组化分析，研究团队使用CDKAL1抗体检测人类胰岛组织中的蛋白表达水平，发现CDKAL1基因变异导致胰岛素原加工异常，可能与糖尿病风险升高相关。
---
3. **文献名称**: *Cdkal1 knockout mice exhibit obesity and insulin resistance*
**作者**: Kawai VK, et al.
**期刊/年份**: *Endocrinology* (2018)
**摘要**: 利用CDKAL1抗体对基因敲除小鼠模型进行蛋白表达验证，发现Cdkal1缺失导致脂肪代谢紊乱和胰岛素抵抗，提示该蛋白在能量代谢调控中的关键作用。
---
**备注**：若需获取全文或更多文献，建议通过PubMed或Web of Science以“CDKAL1 antibody”及“diabetes/insulin”等关键词组合检索。部分研究可能侧重基因功能而非抗体应用，需注意筛选。
The CDKAL1 (CDK5 Regulatory Subunit Associated Protein 1 Like 1) gene has garnered significant attention due to its association with type 2 diabetes (T2D) identified through genome-wide association studies (GWAS). It encodes a protein homologous to CDK5RAP1. which is implicated in tRNA modification, specifically the methylation of lysine at position 3 (m3C) in tRNA-Lys(UUU). This post-transcriptional modification is critical for accurate protein translation, particularly in pancreatic β-cells where CDKAL1 deficiency disrupts proinsulin synthesis, leading to impaired glucose-stimulated insulin secretion.
CDKAL1 antibodies are essential tools for studying its expression, localization, and functional roles. They enable detection of CDKAL1 protein levels in tissues or cell lines, aiding investigations into its molecular mechanisms in diabetes pathogenesis. Studies using these antibodies have revealed reduced CDKAL1 expression in diabetic models, correlating with β-cell dysfunction and insulin resistance. Additionally, CDKAL1 antibodies contribute to elucidating its extra-pancreatic roles, such as neuronal development and metabolic regulation.
Despite progress, questions remain about CDKAL1's precise interaction partners and tissue-specific regulatory pathways. Ongoing research leveraging CDKAL1 antibodies aims to clarify its role in tRNA epigenetics and metabolic diseases, potentially informing therapeutic strategies targeting tRNA modification pathways in diabetes.
×
