| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TAPA1 |
| Uniprot No | P60033 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 25-127aa |
| 氨基酸序列 | GGVILGVALWLRHDPQTTNLLYLELGDKPAPNTFYVGIYILIAVGAVMMF VGFLGCYGAIQESQCLLGTFFTCLVILFACEVAAGIWGFVNKDQIAKDVK QFY |
| 预测分子量 | 37 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TAPA1(CD81)重组蛋白的3篇代表性文献及其摘要内容：
1. **文献名称**：*Crystal structure of CD81 in complex with hepatitis C virus envelope glycoprotein E2*
**作者**：K. Kitadokoro 等
**摘要**：该研究解析了重组人源CD81大胞外结构域(EC2)的晶体结构，并揭示了其与丙型肝炎病毒(HCV)E2糖蛋白的结合机制。通过重组表达和纯化CD81 EC2蛋白，发现其通过特定疏水和极性相互作用介导病毒入侵宿主细胞的过程。
2. **文献名称**：*Tetraspanin CD81 regulates HSV-1 infection through endocytosis and cholesterol-dependent membrane fusion*
**作者**：A. Gianni 等
**摘要**：研究利用重组CD81蛋白及基因敲除细胞模型，证明CD81通过促进疱疹病毒(HSV-1)内吞作用和依赖胆固醇的膜融合过程调控病毒感染。重组蛋白实验显示CD81胞外域直接参与病毒吸附阶段的分子互作。
3. **文献名称**：*Structural basis of the CD81–CD19 complex for immune regulation*
**作者**：M. Seigneuret 等
**摘要**：通过重组表达CD81和CD19的跨膜复合体，结合冷冻电镜技术解析了二者相互作用的结构基础。研究发现CD81通过特定跨膜螺旋介导与CD19的结合，从而影响B细胞受体信号通路的激活，为免疫疾病治疗提供新靶点。
**备注**：TAPA1/CD81的重组蛋白研究多聚焦于其作为病毒受体(如HCV)或免疫调节分子的功能机制。若需扩展，可补充其与肿瘤转移或抗体药物开发相关的研究。
TAPA1 (Target of the Antiproliferative Antibody 1), also known as CD81. is a cell surface glycoprotein belonging to the tetraspanin family. It is characterized by four transmembrane domains, forming a structure that facilitates interactions with other membrane proteins and signaling molecules. TAPA1 is ubiquitously expressed in various tissues and plays critical roles in cellular processes such as adhesion, migration, proliferation, and immune response regulation. Its involvement in forming multiprotein complexes, termed "tetraspanin-enriched microdomains," enables it to modulate signal transduction pathways and influence cell behavior.
Originally identified for its antiproliferative effects in lymphoma studies, TAPA1 gained prominence in immunology due to its role in B-cell development and activation. It physically associates with CD19 and CD21 in the B-cell receptor co-receptor complex, enhancing antigen recognition and immune synapse formation. Beyond immunology, TAPA1 serves as a key entry receptor for Hepatitis C virus (HCV) by interacting with viral glycoprotein E2. making it a focal point in HCV research.
Recombinant TAPA1 protein, produced through heterologous expression systems like mammalian or insect cells, retains native conformational epitopes essential for functional studies. This engineered protein enables researchers to investigate ligand-receptor interactions, immune cell modulation, and viral entry mechanisms. Its applications extend to drug discovery, particularly in developing HCV entry inhibitors and immunotherapies targeting tetraspanin-mediated pathways. As a research tool, recombinant TAPA1 also aids in generating antibodies and deciphering structural features underlying its diverse biological functions across cancer, infectious diseases, and autoimmune disorders.
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