| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/500 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Adenylate cyclase type 8; EC 4.6.1.1; Adenylate cyclase type VIII; ATP pyrophosphate-lyase 8; Adenylyl cyclase 8 |
| Entrez GeneID | 114; |
| WB Predicted band size | 140kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from internal of human ADCY8. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ADCY8抗体的3篇参考文献示例(文献信息为虚构示例，仅供格式参考)：
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1. **文献名称**：*ADCY8 Expression in Hippocampal Neurons and Its Role in Memory Formation*
**作者**：Smith J, et al.
**摘要**：研究通过ADCY8抗体进行免疫组织化学分析，发现ADCY8在小鼠海马神经元中高表达，并证明其通过调控cAMP信号通路参与长期记忆形成。
2. **文献名称**：*ADCY8 Antibody Validation for Detecting Somatic Mutations in Colorectal Cancer*
**作者**：Li X, et al.
**摘要**：研究利用特异性ADCY8抗体进行Western blot和免疫荧光实验，验证其在结直肠癌组织中的表达异常，并发现ADCY8突变与患者预后不良相关。
3. **文献名称**：*ADCY8 Isoform-Specific Localization in Pancreatic β-Cells Using Monoclonal Antibodies*
**作者**：Garcia R, et al.
**摘要**：开发了一种高特异性ADCY8单克隆抗体，通过免疫沉淀和质谱分析揭示了ADCY8在胰岛β细胞中的亚细胞定位及其在胰岛素分泌中的调控作用。
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注：以上文献为模拟内容，实际研究中请通过PubMed、Google Scholar等平台检索真实文献。
The ADCY8 antibody targets adenylate cyclase 8 (ADCY8), a membrane-associated enzyme critical for catalyzing the conversion of ATP to cyclic AMP (cAMP), a key secondary messenger in cellular signaling. ADCY8 belongs to the adenylate cyclase family, which regulates diverse physiological processes, including hormone response, neurotransmission, and cell proliferation. It is particularly enriched in the brain, pancreas, and cardiac tissues, with roles in memory formation (via hippocampal pathways) and insulin secretion in pancreatic β-cells. Dysregulation of ADCY8 has been implicated in neurological disorders, diabetes, and certain cancers.
The ADCY8 antibody is widely used in research to study the protein’s expression, localization, and function. It aids in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to visualize ADCY8 in tissue or cell samples. Antibodies targeting specific epitopes (e.g., C-terminal or N-terminal regions) help distinguish ADCY8 from other adenylate cyclase isoforms. Validation often includes knockout cell lines to confirm specificity.
Structurally, ADCY8 contains 12 transmembrane domains and two cytoplasmic catalytic domains. Its activity is modulated by calcium/calmodulin, linking it to calcium-signaling pathways. Research using ADCY8 antibodies has advanced understanding of cAMP-driven pathways in disease mechanisms, such as β-cell dysfunction in type 2 diabetes or aberrant signaling in tumorigenesis. Commercial ADCY8 antibodies vary by host species, clonality, and conjugation, enabling flexibility in experimental design.
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