| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Adenylate cyclase; type IV; Adenylyl cyclase 4; ATP pyrophosphate-lyase 4; EC 4.6.1.1 |
| Entrez GeneID | 196883; |
| WB Predicted band size | 120kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from internal of human ADCY4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是3篇关于ADCY4抗体的示例参考文献(注：以下内容为示例性虚构文献，实际文献请通过学术数据库查询)：
---
1. **文献名称**：*Role of ADCY4 in Cardiac Hypertrophy: Antibody-Based Expression Analysis*
**作者**：Zhang L, et al.
**摘要**：本研究利用特异性ADCY4抗体，通过Western blot和免疫组化技术，分析了ADCY4在心肌肥大小鼠模型中的表达变化。结果显示，ADCY4在压力超负荷诱导的心肌细胞中显著上调，提示其可能通过调节cAMP信号通路参与心脏重塑。
---
2. **文献名称**：*ADCY4 Antibody Validation for Neuronal Localization Studies*
**作者**：Tanaka K, et al.
**摘要**：该研究验证了一种新型ADCY4多克隆抗体的特异性，并应用于大鼠脑组织的免疫荧光染色。结果显示ADCY4在海马体和前额叶皮层神经元中高表达，支持其在突触可塑性和认知功能中的潜在作用。
---
3. **文献名称**：*ADCY4 Expression in Colorectal Cancer: Diagnostic Implications*
**作者**：Wang Y, et al.
**摘要**：通过ADCY4抗体对结直肠癌组织进行免疫组化分析，发现ADCY4在肿瘤组织中的表达显著高于正常组织，且与患者预后不良相关。研究提示ADCY4可能作为潜在的癌症生物标志物。
---
**建议**：如需真实文献，可通过PubMed或Google Scholar搜索关键词“ADCY4 antibody”、“ADCY4 expression”或“ADCY4 function”，筛选涉及抗体应用(如WB、IHC)的研究。注意结合研究领域(如心血管、神经科学或癌症)进一步缩小范围。
The ADCY4 antibody is a crucial tool for studying adenylate cyclase 4 (ADCY4), a membrane-bound enzyme belonging to the adenylate cyclase family, which catalyzes the conversion of ATP to cyclic AMP (cAMP). ADCY4 plays a pivotal role in intracellular signaling by regulating cAMP levels, thereby influencing diverse physiological processes such as cell proliferation, differentiation, and hormonal response. It is particularly significant in cardiovascular and neuronal systems, where cAMP-mediated pathways modulate heart rate, vascular tone, and synaptic plasticity. Dysregulation of ADCY4 has been implicated in pathologies including heart failure, hypertension, and neurological disorders.
The ADCY4 antibody is typically developed using immunogens derived from specific regions of the ADCY4 protein, such as its cytoplasmic domains, to ensure target specificity. It is widely utilized in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect ADCY4 expression, localization, and abundance in tissues or cell lines. Validated antibodies show minimal cross-reactivity with other adenylate cyclase isoforms (e.g., ADCY3 or ADCY6), ensuring reliable experimental outcomes. Researchers employ this antibody to explore ADCY4’s role in disease mechanisms, drug responses, or genetic models (e.g., knockout mice). Its application also extends to clinical studies, where altered ADCY4 expression may serve as a biomarker or therapeutic target. Proper validation via siRNA knockdown or ADCY4-deficient controls is critical to confirm antibody reliability.
×
