| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/500 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Mediator of RNA polymerase II transcription subunit 23; Mediator complex subunit 23; CRSP complex subunit 3; Cofactor required for Sp1 transcriptional activation subunit 3; Transcriptional coactivator CRSP130 |
| Entrez GeneID | 9439; |
| WB Predicted band size | 150kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from internal of human MED23. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于MED23抗体的参考文献及其简要摘要：
1. **文献名称**：*MED23 regulates EGFR signaling and promotes the proliferation of colorectal cancer cells*
**作者**：Li, X., et al.
**摘要**：该研究利用MED23抗体探究其在结直肠癌细胞中调控EGFR信号通路的作用，发现MED23通过增强MAPK通路活性促进肿瘤增殖。
2. **文献名称**：*MED23 deficiency disrupts the transcription factor network required for neuronal differentiation*
**作者**：Hashimoto, K., et al.
**摘要**：通过MED23抗体检测基因敲除模型，发现MED23缺失破坏神经元分化相关的转录因子网络，影响胚胎干细胞向神经元谱系分化。
3. **文献名称**：*MED23 mediates oncogenic dependencies in triple-negative breast cancer*
**作者**：Zhang, Y., et al.
**摘要**：研究采用MED23抗体进行ChIP-seq分析，揭示MED23在三阴性乳腺癌中通过结合特定转录因子(如AP-1)驱动肿瘤生长和转移。
4. **文献名称**：*Structural basis of MED23 interaction with VP16 and E1A for transcriptional activation*
**作者**：Yang, F., et al.
**摘要**：通过免疫共沉淀(使用MED23抗体)和结构生物学手段，阐明MED23与病毒转录激活因子(如HSV VP16)的相互作用机制，解析其在转录起始中的分子基础。
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**注**：以上文献为示例，实际引用时建议通过PubMed或Web of Science核对最新研究。
The MED23 antibody is a tool used to detect the MED23 protein, a key component of the Mediator complex—a multi-subunit assembly that bridges transcription factors and RNA polymerase II to regulate gene expression. The Mediator complex, comprising approximately 30 subunits in humans, plays a critical role in modulating transcription initiation and elongation. MED23 (Mediator Subunit 23) specifically interacts with transcription factors such as c-Fos and ETS family members, influencing pathways involved in cell growth, differentiation, and stress responses. Dysregulation of MED23 has been linked to diseases, including cancers and neurological disorders, due to its role in oncogenic signaling and neural development.
MED23 antibodies are widely used in research to study protein expression, localization, and molecular interactions via techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP). These antibodies help elucidate MED23's functional mechanisms in gene regulation and its association with pathological conditions. Commercial MED23 antibodies are typically raised in hosts like rabbits or mice, targeting specific epitopes of the human MED23 protein. Validation for specificity and sensitivity is crucial, as non-specific binding can arise due to the Mediator complex's structural complexity. Overall, MED23 antibodies are vital for advancing understanding of transcriptional regulation and disease mechanisms.
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