| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Transcription factor-like 5 protein; Cha transcription factor; HPV-16 E2-binding protein 1; E2BP-1; TCFL5 |
| Entrez GeneID | 10732; |
| WB Predicted band size | 48kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from internal of human TCFL5. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于TCFL5抗体的3篇参考文献示例(注：文献信息为模拟生成，仅供参考)：
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1. **文献名称**：*TCFL5 regulates spermatogonial stem cell maintenance through transcriptional activation in mouse testes*
**作者**：Zhang Y, et al.
**摘要**：本研究利用TCFL5特异性抗体，通过免疫组化和Western blot分析，揭示了TCFL5在小鼠精原干细胞中的关键作用，证明其通过调控下游靶基因(如PLZF)维持干细胞自我更新。
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2. **文献名称**：*TCFL5 antibody-based ChIP-seq reveals its role in DNA repair pathways in ovarian cancer cells*
**作者**：Wang L, et al.
**摘要**：通过TCFL5抗体的染色质免疫沉淀测序(ChIP-seq)，作者发现TCFL5在卵巢癌细胞中结合DNA损伤修复相关基因启动子区域，提示其可能通过调控修复机制影响化疗耐药性。
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3. **文献名称**：*TCFL5 modulates pluripotency in human embryonic stem cells via interaction with OCT4*
**作者**：Kim S, et al.
**摘要**：研究采用TCFL5抗体进行免疫共沉淀(Co-IP)实验，证实TCFL5与OCT4蛋白相互作用，并通过调控多能性基因(如NANOG)表达影响人胚胎干细胞的自我更新能力。
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**备注**：上述文献为示例，实际研究中请通过PubMed或Google Scholar以“TCFL5 antibody”及“TCFL5 function”等关键词检索最新文献。部分研究可能未在摘要中明确提及抗体使用细节，需结合方法部分进一步确认。
The TCFL5 (Transcription Factor Like 5) antibody is a tool used to study the TCFL5 protein, a member of the basic helix-loop-helix (bHLH) transcription factor family. TCFL5. also known as CHIC (Chromatin, Cell Cycle, and Cancer-associated protein), is primarily expressed in germ cells and plays a critical role in spermatogenesis. It regulates gene expression during meiosis and post-meiotic stages, influencing chromatin remodeling, transcriptional activation, and germ cell differentiation. Studies highlight its interaction with MYBL1 and involvement in maintaining the balance between proliferation and differentiation in male germ cells.
TCFL5 antibodies are widely utilized in reproductive biology and oncology research. They enable detection of TCFL5 expression in testicular tissues or germ cell-derived cell lines via techniques like Western blot, immunohistochemistry, and immunofluorescence. These antibodies help elucidate TCFL5's role in fertility and its potential dysregulation in conditions like azoospermia or germ cell tumors. Commercially available TCFL5 antibodies are typically validated for specificity in human, mouse, or rat samples, with applications spanning basic research and diagnostic exploration. Recent studies also investigate its broader regulatory networks, linking TCFL5 to epigenetic modifications and cell cycle control in cancer contexts.
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