| 纯度 | ＞90% by SDS-PAGE. |
| 种属 | Human |
| 靶点 | SFN |
| Uniprot No | P31947 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-248aa |
| 氨基酸序列 | MERASLIQKAKLAEQAERYEDMAAFMKGAVEKGEELSCEERNLLSVAYKNVVGGQRAAWRVLSSIEQKSNEEGSEEKGPEVREYREKVETELQGVCDTVLGLLDSHLIKEAGDAESRVFYLKMKGDYYRYLAEVATGDDKKRIIDSARSAYQEAMDISKKEMPPTNPIRLGLALNFSVFHYEIANSPEEAISLAKTTFDEAMADLHTLSEDSYKDSTLIMQLLRDNLTLWTADNAGEEGGEAPQEPQS |
| 预测分子量 | 62.9 kDa |
| 蛋白标签 | C-his |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是为您整理的3篇关于SFN(Stratifin/14-3-3σ)重组蛋白研究的示例参考文献格式(注：文献标题和作者为虚构示例，建议通过PubMed或Web of Science查询真实文献)：
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1. **标题**: *High-yield production of recombinant human SFN protein in E. coli and its functional characterization*
**作者**: Li X, Wang Y, Chen Z
**摘要**: 本研究报道了通过大肠杆菌表达系统高效表达人源SFN重组蛋白的优化方法，利用His标签纯化获得高纯度蛋白，并通过体外实验验证了SFN对细胞周期蛋白CDK1的磷酸化调控作用。
2. **标题**: *SFN recombinant protein inhibits tumor growth by modulating p53 pathway in breast cancer models*
**作者**: Gupta R, et al.
**摘要**: 通过哺乳动物细胞表达系统制备SFN重组蛋白，体内实验证明其能通过激活p53信号通路抑制乳腺癌细胞增殖，为靶向治疗提供了潜在策略。
3. **标题**: *Structural analysis of SFN-protein interactions via recombinant mutant screening*
**作者**: Tanaka K, et al.
**摘要**: 利用重组SFN点突变体库结合表面等离子体共振(SPR)技术，鉴定了SFN与伴侣蛋白Bax的结合关键结构域，揭示了其在线粒体凋亡途径中的分子机制。
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如需具体文献，建议通过以下途径查询：
1. **PubMed**(https://pubmed.ncbi.nlm.nih.gov/)搜索关键词：`"recombinant SFN protein"` 或 `"14-3-3 sigma recombinant"`
2. **Google Scholar** 高级检索：`recombinant AND (SFN OR Stratifin OR 14-3-3σ)`
3. 关注领域内权威期刊如*Protein Expression and Purification*、*Journal of Biological Chemistry*等。
**Background of SFN Recombinant Protein**
Stratifin (SFN), also known as 14-3-3 sigma, is a multifunctional protein encoded by the *SFN* gene, belonging to the 14-3-3 protein family. It plays a critical role in regulating cellular processes such as cell cycle progression, apoptosis, DNA damage response, and signal transduction. SFN acts as an adaptor molecule, binding to phosphorylated serine/threonine residues on target proteins to modulate their activity, localization, or stability. Notably, it interacts with key regulators like p53. CDC2. and CDK1. linking it to tumor suppression and oncogenesis.
SFN gained prominence due to its association with cancer biology. It is frequently downregulated in malignancies, including breast, gastric, and liver cancers, often via promoter hypermethylation. This loss correlates with poor prognosis, suggesting its role as a tumor suppressor. Conversely, SFN overexpression in certain contexts may promote chemoresistance, highlighting its complex duality.
Recombinant SFN protein is produced using biotechnological platforms (e.g., *E. coli* or mammalian expression systems) to ensure high purity and bioactivity. Its production enables mechanistic studies, such as elucidating interactions with binding partners or exploring its role in epithelial cell polarity and differentiation. Additionally, recombinant SFN serves as a tool for developing diagnostic assays or therapeutic strategies, including cancer biomarkers or targeted therapies.
Research on SFN recombinant protein continues to expand, driven by its potential in understanding cancer progression, improving clinical outcomes, and advancing personalized medicine. Its dual role in tumorigenesis underscores the need for context-specific studies to harness its therapeutic value effectively.
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