| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Forkhead box protein D3; HNF3/FH transcription factor genesis; FOXD3; |
| Entrez GeneID | 27022; |
| WB Predicted band size | 48kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from internal of human FOXD3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3-4条关于FOXD3抗体的模拟参考文献示例(实际文献需通过学术数据库查询)：
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1. **标题**：FOXD3 regulates neural crest development through transcriptional repression
**作者**：Smith J, Doe R, Lee C
**摘要**：研究利用FOXD3特异性抗体，通过染色质免疫沉淀(ChIP)和免疫荧光技术，揭示了FOXD3在神经嵴细胞分化中的转录抑制作用，证明其通过调控靶基因维持胚胎发育的时序性。
2. **标题**：FOXD3 antibody validation in melanoma cell lines
**作者**：Brown K, Wilson T
**摘要**：该研究系统验证了FOXD3抗体在Western blot和免疫组化中的特异性，发现FOXD3在黑色素瘤中表达显著下调，提示其作为肿瘤抑制因子的潜在作用。
3. **标题**：FOXD3 maintains pluripotency in embryonic stem cells
**作者**：Zhang L, Chen H
**摘要**：通过FOXD3抗体介导的敲低实验，研究发现FOXD3通过抑制分化相关基因(如SOX2和OCT4)的表观遗传修饰，维持胚胎干细胞的多能性。
4. **标题**：FOXD3 expression correlates with cancer metastasis
**作者**：Garcia M, Patel S
**摘要**：利用FOXD3抗体对临床组织样本分析发现，FOXD3低表达与乳腺癌和结直肠癌的转移能力呈负相关，机制涉及EMT通路调控。
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**注**：以上为模拟示例，实际文献需通过PubMed/Google Scholar等平台以关键词“FOXD3 antibody”或“FOXD3 function”检索近年研究。
FOXD3 (Forkhead Box D3) is a transcription factor belonging to the Forkhead family, characterized by a conserved winged-helix DNA-binding domain. It plays critical roles in embryonic development, stem cell pluripotency, and differentiation by regulating gene expression networks. FOXD3 is particularly noted for its function in neural crest development, where it maintains progenitor cell multipotency and suppresses premature differentiation. In cancer biology, FOXD3 exhibits dual roles, acting as a tumor suppressor in some contexts (e.g., melanoma, neuroblastoma) by repressing pro-invasive genes, while promoting tumor progression in others (e.g., breast cancer) through mechanisms not fully understood.
FOXD3 antibodies are essential tools for studying its expression, localization, and molecular interactions. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP) to explore FOXD3's regulatory roles. Researchers also employ FOXD3 antibodies to investigate its involvement in cancer metastasis, stem cell maintenance, and developmental disorders. Commercial FOXD3 antibodies are typically raised against specific epitopes within its N-terminal or DNA-binding domains, with validation across species (human, mouse, rat). Due to its context-dependent roles, FOXD3 remains a focus in regenerative medicine and oncology, driving demand for reliable antibodies to advance diagnostic and therapeutic strategies.
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