Rabbit Polyclonal CNOT7 Antibody

The CNOT7 antibody is a crucial tool for studying the CCR4-NOT complex, a highly conserved multiprotein assembly central to post-transcriptional gene regulation in eukaryotes. CNOT7 (also known as CAF1) is a catalytic subunit of this complex, functioning as a deadenylase that shortens mRNA poly(A) tails—a critical step in mRNA turnover and translation control. It interacts with other subunits like CNOT6/6L and CNOT8. contributing to diverse cellular processes including transcriptional regulation, miRNA-mediated silencing, and DNA damage response. Researchers employ CNOT7 antibodies primarily to investigate its expression patterns, subcellular localization, and protein-protein interactions via techniques like Western blotting, immunoprecipitation, and immunofluorescence. These studies are vital for understanding its role in cellular homeostasis, embryonic development, and diseases such as cancer, where CNOT7 dysregulation has been linked to tumor progression and metastasis. The antibody's specificity and sensitivity are rigorously validated using knockout controls to ensure accurate detection of endogenous CNOT7. which typically migrates at ~35-40 kDa. Recent applications include exploring CNOT7's involvement in stress granule formation, viral infection responses, and metabolic pathways, highlighting its broad impact on cellular physiology.