关键信息
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基因名
KLHL29
- 应用
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别名
KBTBD9; Kelch like protein 29; kelch repeat and BTB (POZ) domain containing 9; Kelch repeat and BTB domain containing protein 9 ; Kelch repeat and BTB domain-containing protein 9; Kelch-like protein 29; KIAA1921; KLH29_HUMAN; KLHL 29; Klhl29
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种属
Human
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表达系统
E. coli
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标签
GST-tag at N-terminal
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q96CT2
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表达区间
1-503aa
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氨基酸序列
MPGHYSLPQPPSQPLSSVVVNMPAQALYASPQPLAVSTLPGVGQVARPGPTAVGNGHMAGPLLPPPPPAQPSATLPSGAPATNGPPTTDSAHGLQMLRTIGVGKYEFTDPGHPREMLKELNQQRRAKAFTDLKIVVEGREFEVHQNVLASCSLYFKDLIQRSVQDSGQGGREKLELVLSNLQADVLELLLEFVYTGSLVIDSANAKTLLEAASKFQFHTFCKVCVSFLEKQLTASNCLGVLAMAEAMQCSELYHMAKAFALQIFPEVAAQEEILSISKDDFIAYVSNDSLNTKAEELVYETVIKWIKKDPATRTQYAAELLAVVRLPFIHPSYLLNVVDNEELIKSSEACRDLVNEAKRYHMLPHARQEMQTPRTRPRLSAGVAEVIVLVGGRQMVGMTQRSLVAVTCWNPQNNKWYPLASLPFYDREFFSVVSAGDNIYLSGGMESGVTLADVWCYMSLLDNWNLVSRMTVPRCRHNSLVYDGKIYTLGGLGVAGNVDHVER
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分子量
81.6 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
KLHL29 is a member of the Kelch-like protein family, characterized by the presence of a BTB (Broad-Complex, Tramtrack, and Bric-a-Brac) domain and multiple Kelch repeats. This protein has garnered attention in recent years due to its potential roles in various cellular processes, including protein degradation, cell signaling, and cytoskeletal organization. Its involvement in diseases, particularly in the context of neurodegenerative disorders and cancer, has further intensified research efforts. Studies have indicated that KLHL29 may act as an E3 ubiquitin ligase, facilitating the ubiquitination and subsequent proteasomal degradation of target proteins, thereby influencing cellular homeostasis. Moreover, aberrations in KLHL29 expression levels have been associated with pathological conditions, underscoring its importance in maintaining cellular functions. The recombinant production of KLHL29 protein is critical for in-depth biochemical and biophysical analyses, enabling researchers to decode its mechanism of action, identify substrate proteins, and explore its therapeutic potential. Understanding the functional implications of KLHL29 at a molecular level could yield insights into its role in health and disease, paving the way for novel intervention strategies. Consequently, extensive studies focusing on the structural properties, interaction networks, and regulatory mechanisms of KLHL29 are essential to elucidate its full biological significance and therapeutic applications.
