| WB | 1/500-1/3000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HMGX1; high-mobility group 20A; |
| Entrez GeneID | 10362; |
| WB Predicted band size | 36kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from N-terminal of human HMG20B. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于HMG20B抗体的虚构参考文献示例(基于典型研究场景，非真实文献)：
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1. **Title**: *HMG20B regulates neurodevelopmental processes through chromatin remodeling*
**Authors**: Smith J, et al. (2020)
**Summary**: 本研究利用特异性HMG20B抗体进行免疫沉淀和Western blot分析，发现HMG20B通过与染色质修饰复合体相互作用调控神经元分化。抗体验证显示其在人脑组织和小鼠模型中高表达。
2. **Title**: *A novel monoclonal antibody for HMG20B detects its oncogenic role in colorectal cancer*
**Authors**: Lee S, et al. (2018)
**Summary**: 报道一种新型HMG20B单克隆抗体的开发，通过免疫组化证实HMG20B在结直肠癌中过表达，并与患者预后不良相关。抗体特异性经siRNA敲低实验验证。
3. **Title**: *HMG20B modulates epigenetic silencing in embryonic stem cells*
**Authors**: Garcia R, et al. (2021)
**Summary**: 使用HMG20B抗体进行ChIP-seq分析，揭示其在胚胎干细胞中靶向多梳抑制复合物(PRC2)，调控关键发育基因的沉默。抗体适用于流式细胞术和免疫荧光。
4. **Title**: *HMG20B antibody-based profiling identifies its role in hepatic differentiation*
**Authors**: Chen X, et al. (2019)
**Summary**: 通过HMG20B抗体介导的蛋白质组学分析，发现其在肝细胞分化中调控Wnt/β-catenin通路。研究验证了抗体在人类原代细胞和类器官模型中的适用性。
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注：以上文献为示例性质，实际研究中请通过学术数据库(如PubMed、Google Scholar)检索真实文献。
The HMG20B antibody is a research tool designed to detect and study the High Mobility Group 20B (HMG20B) protein, a member of the HMG-box superfamily. HMG20B, also known as BRAF35-homologous (BRD7HB), is a chromatin-associated protein involved in transcriptional regulation and chromatin remodeling. It plays critical roles in cellular processes such as neuronal differentiation, cell cycle progression, and epigenetic modulation. HMG20B interacts with histone deacetylase (HDAC) complexes and other chromatin modifiers, influencing gene expression by altering chromatin structure. Its function is particularly significant in neurodevelopment, where it regulates the transition from neural stem cells to differentiated neurons. Dysregulation of HMG20B has been implicated in cancer, neurodegenerative disorders, and neurodevelopmental conditions like autism spectrum disorders.
The HMG20B antibody is widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to investigate protein expression, localization, and interactions. Researchers rely on its specificity to explore HMG20B's role in gene silencing, differentiation pathways, and disease mechanisms. Validated antibodies are essential for distinguishing HMG20B from related HMG-box proteins, such as HMG20A, due to structural similarities. Recent studies using HMG20B antibodies have highlighted its potential as a therapeutic target in cancers with epigenetic dysregulation. Proper antibody validation via knockout controls or siRNA knockdown is crucial to ensure accuracy in experimental models.
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