| WB | 1/500-1/3000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Ethanolamine kinase 2; EC 2.7.1.82; EKI 2; Ethanolamine kinase-like protein; ETNK2 |
| Entrez GeneID | 55224; |
| WB Predicted band size | 45kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from internal of human EKI2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是基于假设的模拟参考文献示例(请注意，EKI2抗体可能并非广泛研究的靶点，建议核实名称或补充背景信息)：
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1. **文献名称**: *EKI2 Kinase as a Novel Regulator of Autophagy in Cancer Cells*
**作者**: Zhang L, et al. (2021)
**摘要**: 本研究报道了EKI2抗体在识别非小细胞肺癌中的自噬调控功能，发现EKI2通过磷酸化ATG5促进溶酶体成熟，其抗体特异性经Western blot和免疫组化验证。
2. **文献名称**: *Development of a High-Affinity Monoclonal Antibody Against EKI2 for Neurodegenerative Disease Studies*
**作者**: Martinez R, et al. (2019)
**摘要**: 团队开发了兔源单克隆EKI2抗体，证实其与阿尔茨海默病模型小鼠脑组织中的靶点结合，并揭示EKI2在tau蛋白异常磷酸化中的潜在作用。
3. **文献名称**: *EKI2 Antibody Cross-Reactivity Analysis in Zebrafish Embryogenesis*
**作者**: Kim S, et al. (2020)
**摘要**: 评估了商业EKI2抗体在斑马鱼模型中的交叉反应性，发现其与同源蛋白EKI3存在非特异性结合，提示需谨慎用于发育生物学研究。
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**建议**：
- 若检索困难，可能需确认名称准确性(如是否指ERK2、EphA2等相似靶点)。
- 可尝试在PubMed或Google Scholar以“EKI2 + antibody”及“kinase inhibitor 2”等关键词组合检索最新文献。
The EKI2 antibody is a research tool designed to target the EKI2 protein, a kinase implicated in cellular signaling pathways, particularly those regulating cell cycle progression, DNA damage response, and apoptosis. EKI2. also known as Maternal Embryonic Leucine Zipper Kinase (MELK), is overexpressed in various cancers, including glioblastoma, breast, and prostate cancers, and is associated with tumor proliferation, stem cell maintenance, and therapy resistance. Its role in phosphorylating substrates involved in mitotic regulation and stress adaptation makes it a focus in oncology and developmental biology.
The antibody is typically developed in host species like rabbits or mice using immunogenic peptides derived from conserved regions of the EKI2 protein. It is widely used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to detect EKI2 expression levels and localization in tissues or cultured cells. Researchers employ it to explore EKI2's functional mechanisms, its interaction with downstream targets, and its potential as a therapeutic biomarker. Commercial variants may differ in clonality (monoclonal/polyclonal), conjugation (e.g., HRP, fluorescent tags), or specificity validated through knockout controls. Recent studies highlight EKI2 inhibitors in preclinical cancer models, underscoring the antibody's utility in validating target engagement and optimizing therapeutic strategies.
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