| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRMT5 |
| Uniprot No | O14744 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-637aa |
| 氨基酸序列 | MAAMAVGGAG GSRVSSGRDL NCVPEIADTL GAVAKQGFDF LCMPVFHPRF KREFIQEPAK NRPGPQTRSD LLLSGRDWNT LIVGKLSPWI RPDSKVEKIR RNSEAAMLQE LNFGAYLGLP AFLLPLNQED NTNLARVLTN HIHTGHHSSM FWMRVPLVAP EDLRDDIIEN APTTHTEEYS GEEKTWMWWH NFRTLCDYSK RIAVALEIGA DLPSNHVIDR WLGEPIKAAI LPTSIFLTNK KGFPVLSKMH QRLIFRLLKL EVQFIITGTN HHSEKEFCSY LQYLEYLSQN RPPPNAYELF AKGYEDYLQS PLQPLMDNLE SQTYEVFEKD PIKYSQYQQA IYKCLLDRVP EEEKDTNVQV LMVLGAGRGP LVNASLRAAK QADRRIKLYA VEKNPNAVVT LENWQFEEWG SQVTVVSSDM REWVAPEKAD IIVSELLGSF ADNELSPECL DGAQHFLKDD GVSIPGEYTS FLAPISSSKL YNEVRACREK DRDPEAQFEM PYVVRLHNFH QLSAPQPCFT FSHPNRDPMI DNNRYCTLEF PVEVNTVLHG FAGYFETVLY QDITLSIRPE THSPGMFSWF PILFPIKQPI TVREGQTICV RFWRCSNSKK VWYEWAVTAP VCSAIHNPTG RSYTIGL |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PRMT5重组蛋白的3篇参考文献示例(注：文献标题与作者为虚构示例，仅供参考格式)：
1. **标题**：*Structural and functional analysis of the human PRMT5-MEP50 complex*
**作者**：Antonelli, S.C., et al.
**摘要**：该研究利用重组表达的PRMT5与MEP50复合物，通过X射线晶体学解析其三维结构，揭示了二者协同催化精氨酸甲基化的分子机制，并验证了重组蛋白的甲基转移酶活性。
2. **标题**：*Development of a high-throughput assay for PRMT5 activity using recombinant protein*
**作者**：Wang, Y., et al.
**摘要**：作者通过大肠杆菌系统表达并纯化重组PRMT5.建立了一种基于荧光偏振的酶活性检测方法，用于快速筛选小分子抑制剂，为靶向PRMT5的药物开发提供工具。
3. **标题**：*PRMT5-dependent methylation of splicing factors regulates cancer cell proliferation*
**作者**：Beketova, M., et al.
**摘要**：研究利用重组PRMT5蛋白体外验证其对剪接因子(如Sm蛋白)的精氨酸甲基化作用，证明该修饰通过调控mRNA剪接促进肿瘤细胞增殖，为癌症治疗提供新靶点。
4. **标题**：*Substrate specificity profiling of recombinant PRMT5 reveals distinct methylation patterns*
**作者**：Zurita-Lopez, C.I., et al.
**摘要**：通过重组PRMT5蛋白与不同底物的体外反应实验，系统分析其甲基化位点偏好性，发现其对精氨酸对称二甲基化的底物选择性受MEP50亚基调控。
(注：以上文献为示例，实际引用时需核实真实性与具体信息。)
**Background of PRMT5 Recombinant Protein**
Protein arginine methyltransferase 5 (PRMT5) is a member of the PRMT family, which catalyzes the symmetric dimethylation of arginine residues on histones and non-histone proteins. This post-translational modification regulates diverse cellular processes, including transcriptional regulation, RNA splicing, DNA repair, and signal transduction. PRMT5 forms a multimeric complex with co-factors such as MEP50 (methylosome protein 50), enhancing its substrate specificity and enzymatic activity. Its targets encompass histones (e.g., H4R3. H3R8) and proteins like Sm proteins in spliceosomes, p53. and EGFR, linking PRMT5 to epigenetic regulation, cell cycle control, and oncogenesis.
Recombinant PRMT5 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), retains enzymatic activity and is widely used in biochemical and structural studies. Its production enables detailed characterization of substrate interactions, methylation kinetics, and inhibitor screening. PRMT5 is implicated in cancer, where its overexpression correlates with poor prognosis in malignancies such as glioblastoma, leukemia, and lung cancer. Dysregulation of PRMT5 disrupts tumor suppressor pathways and promotes cell proliferation, making it a therapeutic target.
Small-molecule inhibitors of PRMT5 (e.g., GSK3326595. EPZ015666) are under clinical evaluation, particularly for cancers with methylthioadenosine phosphorylase (MTAP) deletion, exploiting synthetic lethality. Recombinant PRMT5 facilitates drug discovery by enabling high-throughput assays and crystallography to optimize inhibitor design. Beyond oncology, PRMT5 is studied in immune regulation, viral replication, and developmental disorders, underscoring its broad biological relevance.
In summary, PRMT5 recombinant protein serves as a critical tool for unraveling methylation-dependent mechanisms and advancing targeted therapies, reflecting its central role in cellular homeostasis and disease.
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