| WB | 1/500-1/3000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/100-1/500 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Dual specificity protein phosphatase 16; EC 3.1.3.48; EC 3.1.3.16; Mitogen-activated protein kinase phosphatase 7; MAP kinase phosphatase 7 |
| Entrez GeneID | 80824; |
| WB Predicted band size | 73kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from internal of human DUSP16. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于DUSP16抗体的模拟参考文献示例(实际文献需通过学术数据库查询验证)：
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1. **文献名称**: *DUSP16/MKP-7 regulates inflammatory responses via modulation of p38 MAPK signaling in macrophages*
**作者**: Yamamoto, K. et al.
**摘要**: 本研究利用特异性DUSP16抗体，通过Western blot和免疫荧光技术，揭示了DUSP16通过负调控p38 MAPK磷酸化抑制巨噬细胞中促炎细胞因子的释放，表明其在炎症性疾病中的潜在治疗作用。
2. **文献名称**: *Expression profiling of DUSP16 in human colorectal cancer and its association with patient prognosis*
**作者**: Chen, L. et al.
**摘要**: 通过免疫组化(使用DUSP16抗体)分析结直肠癌组织样本，发现DUSP16低表达与肿瘤进展和患者生存率降低显著相关，提示其作为预后生物标志物的可能性。
3. **文献名称**: *DUSP16 modulates neuronal apoptosis via JNK signaling in Alzheimer’s disease models*
**作者**: Park, S. et al.
**摘要**: 在小鼠阿尔茨海默病模型中，采用DUSP16抗体检测发现其表达下调，并通过激活JNK通路促进神经元凋亡，为神经退行性疾病的机制研究提供了新视角。
4. **文献名称**: *Development and validation of a novel monoclonal antibody against human DUSP16 for functional studies*
**作者**: Rodriguez, A. et al.
**摘要**: 本文报道了一种高特异性抗人DUSP16单克隆抗体的开发与验证，通过流式细胞术和免疫沉淀实验证实其适用于多种细胞类型的DUSP16蛋白功能研究。
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**注意**：以上为模拟示例，真实文献需通过PubMed、Google Scholar等平台检索关键词如“DUSP16 antibody”“MKP-7”并结合实验应用场景(如Western blot、IHC)获取。建议使用学术数据库查询最新研究。
The DUSP16 antibody is a crucial tool for studying dual-specificity phosphatase 16 (DUSP16), also known as mitogen-activated protein kinase phosphatase-7 (MKP-7). DUSP16 belongs to the dual-specificity phosphatase (DUSP) family, which regulates mitogen-activated protein kinase (MAPK) signaling pathways by dephosphorylating both tyrosine and threonine residues. Specifically, DUSP16 inactivates stress-activated MAPKs, such as JNK and p38. influencing cellular responses to environmental stress, inflammation, and apoptosis. It is expressed in various tissues, including the brain, immune cells, and endocrine organs, and plays roles in immune regulation, neuronal development, and metabolic processes.
Research on DUSP16 has linked it to diseases such as cancer, neurodegenerative disorders, and autoimmune conditions. For example, its dysregulation may contribute to tumor progression or chemoresistance by modulating MAPK-dependent pathways. In neuroscience, DUSP16 is implicated in synaptic plasticity and neuronal survival, with potential relevance to Alzheimer’s and Parkinson’s diseases.
The DUSP16 antibody enables detection and quantification of this phosphatase in experimental models, facilitating studies on its expression patterns, subcellular localization, and interactions. Antibodies are validated for techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Researchers must select antibodies based on species reactivity, epitope specificity, and application compatibility. Proper validation ensures accurate results, particularly given the structural homology among DUSP family members. Commercial antibodies are typically developed in hosts like rabbits or mice, often with tags (e.g., recombinant His-tagged proteins) for enhanced detection.
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