| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ART3 |
| Uniprot No | Q13508 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-362aa |
| 氨基酸序列 | MKTGHFEIVT MLLATMILVD IFQVKAEVLD MADNAFDDEY LKCTDRMEIK YVPQLLKEEK ASHQQLDTV WENAKAKWAA RKTQIFLPMN FKDNHGIALM AYISEAQEQT PFYHLFSEAV KMAGQSRED YIYGFQFKAF HFYLTRALQL LRKPCEASSK TVVYRTSQGT SFTFGGLNQA RFGHFTLAY SAKPQAANDQ LTVLSIYTCL GVDIENFLDK ESERITLIPL NEVFQVSQEG AGNNLILQS INKTCSHYEC AFLGGLKTEN CIENLEYFQP IYVYNPGEKN QKLEDHSEKN WKLEDHGEK NQKLEDHGVK ILEPTQIPGM KIPEPFPLPE DKSQGNINNP TPGPVPVPGP KSHPSAS |
| 预测分子量 | 40 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ART3重组蛋白的参考文献及摘要概览：
1. **文献名称**：*"Characterization of human ART3: a mono-ADP-ribosyltransferase involved in immune cell signaling"*
**作者**：Okazaki IJ et al.
**摘要**：研究报道了人源ART3重组蛋白在大肠杆菌中的表达与纯化，证实其具有单ADP-核糖基转移酶活性，并参与T细胞受体信号通路的调控。
2. **文献名称**：*"Functional analysis of ART3 in DNA damage response via recombinant protein expression"*
**作者**：Li H et al.
**摘要**：通过哺乳动物细胞系统表达ART3重组蛋白，发现其与PARP1相互作用，可能通过ADP-核糖基化修饰影响DNA损伤修复过程。
3. **文献名称**：*"Structural insights into ART3 catalysis: Crystal structure of recombinant mouse ART3"*
**作者**：Deng Y et al.
**摘要**：解析了小鼠ART3重组蛋白的晶体结构，揭示其催化域的关键氨基酸残基及底物结合模式，为设计靶向抑制剂奠定基础。
4. **文献名称**：*"ART3 recombinant protein suppresses tumor growth by modulating NAD+ metabolism in cancer models"*
**作者**：Wang X et al.
**摘要**：在体外实验中，重组ART3蛋白通过消耗NAD+抑制肿瘤细胞增殖，并在小鼠模型中表现出抗肿瘤活性，提示其潜在治疗价值。
注：上述文献信息为示例性质，实际引用时需核实具体来源及内容准确性。
ART3 (ADP-ribosyltransferase 3) is a member of the ecto-ADP-ribosyltransferase family, enzymes that catalyze the transfer of ADP-ribose groups from NAD⁺ to target proteins, modulating their activity. Initially identified in human tissues, ART3 is a glycosylphosphatidylinositol (GPI)-anchored cell surface protein predominantly expressed in testis, brain, and immune cells. Its biological role remains less characterized compared to other ART family members (e.g., ART1 or ART5), but emerging evidence suggests involvement in cell signaling, immune regulation, and cancer progression.
The ART3 gene encodes a protein with conserved catalytic motifs critical for ADP-ribosylation, though its enzymatic activity in vivo remains debated. Structural studies reveal a conserved "R-S-EXE" motif essential for NAD⁺ binding. Unlike other ARTs, ART3 may function through non-enzymatic mechanisms, potentially acting as a receptor or adhesion molecule. In cancer, ART3 overexpression correlates with tumor invasiveness and poor prognosis, possibly by influencing pathways like Wnt/β-catenin or EGFR signaling. In the nervous system, it may participate in neuroprotection or synaptic plasticity.
Recombinant ART3 protein is typically produced in mammalian expression systems (e.g., HEK293 cells) to preserve post-translational modifications, followed by affinity chromatography purification. Its applications include functional studies to resolve controversies around catalytic activity, antibody development, and screening for therapeutic inhibitors. Research challenges include identifying physiological substrates and clarifying ART3's dual roles in normal physiology versus disease contexts. Recent interest focuses on its potential as a biomarker or target in cancers and neurodegenerative disorders.
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