| WB | 1/500-1/3000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ADP-dependent glucokinase; EC 2.7.1.147; ADPGK; ADP-GK; RbBP-35 |
| Entrez GeneID | 83440; |
| WB Predicted band size | 47kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from internal of human ADPGK. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ADPGK(ADP-dependent glucokinase)抗体的3篇参考文献示例(注：文献为虚拟示例，实际文献需通过数据库检索确认)：
1. **文献名称**：*ADP-dependent glucokinase as a novel therapeutic target in glioblastoma*
**作者**：Mathupala SP, et al.
**摘要**：该研究揭示了ADPGK在胶质母细胞瘤中的高表达，并开发了一种兔源多克隆抗体，用于Western blot和免疫组化检测肿瘤组织中ADPGK的活性及定位，提示其作为潜在治疗靶点的价值。
2. **文献名称**：*Characterization of a monoclonal antibody against human ADPGK and its application in metabolic studies*
**作者**：Chen L, et al.
**摘要**：文章报道了一种高特异性小鼠单克隆抗体的制备，通过ELISA和免疫荧光验证其结合能力，并应用于肝癌细胞系中ADPGK的糖酵解功能研究，证实其在能量代谢中的关键作用。
3. **文献名称**：*ADPGK expression correlates with cancer progression: Insights from a pan-cancer antibody-based analysis*
**作者**：Kim JH, et al.
**摘要**：研究利用商业化ADPGK抗体(货号AB123.ABC公司)对多种癌症组织进行蛋白表达分析，发现ADPGK在结直肠癌和肺癌中显著上调，提示其作为预后标志物的可能性。
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如需真实文献，建议通过PubMed或Google Scholar检索关键词“ADPGK antibody”或“ADP-dependent glucokinase antibody”。
The ADP-dependent glucokinase (ADPGK) antibody is a research tool designed to detect and study ADPGK, an enzyme involved in alternative glycolytic pathways. ADPGK catalyzes the phosphorylation of glucose using ADP instead of ATP, a mechanism observed in certain archaea, protozoa, and mammalian cells under metabolic stress. Its role in cancer metabolism has garnered attention, as ADPGK is upregulated in some tumors, suggesting a potential link to the Warburg effect and tumor survival in hypoxic conditions.
Polyclonal or monoclonal ADPGK antibodies are typically generated using immunogenic peptides derived from conserved regions of the human ADPGK protein (e.g., C-terminal or N-terminal domains). These antibodies enable the detection of ADPGK expression via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Validation often includes testing on cell lines with known ADPGK expression or knockout models to confirm specificity.
Research applications focus on understanding ADPGK's metabolic contributions in diseases, particularly cancer and infections. For instance, studies explore its role in *Plasmodium* species, where ADP-dependent kinases are essential for energy production. In oncology, ADPGK inhibition is investigated for therapeutic potential. However, the antibody's utility depends on rigorous validation due to potential cross-reactivity with homologous proteins. Overall, ADPGK antibodies serve as critical tools for dissecting non-canonical glycolysis and its implications in pathophysiology.
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