| WB | 1/500-1/3000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Forkhead box protein B1/2; Transcription factor FKH-5; FOXB1/2; FKH5; |
| Entrez GeneID | 27023; |
| WB Predicted band size | 45kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from internal of human FOXB1/2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于FOXB1/2抗体的参考文献示例(请注意，以下内容为模拟生成，实际文献需通过学术数据库查询)：
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1. **文献名称**: *"Characterization of FOXB1 Antibody in Neural Stem Cell Differentiation"*
**作者**: Smith A, et al.
**摘要**: 本研究开发了一种特异性识别FOXB1蛋白的单克隆抗体，通过免疫印迹和免疫荧光验证其在神经干细胞分化中的表达模式。结果显示FOXB1在早期神经分化阶段显著上调，抗体可用于体外和体内模型的功能研究。
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2. **文献名称**: *"FOXB2 as a Tumor Suppressor: Validation of a Polyclonal Antibody in Cancer Models"*
**作者**: Lee H, et al.
**摘要**: 文章报道了一种高特异性兔源多克隆FOXB2抗体的制备，并在乳腺癌和肺癌组织样本中验证其表达。研究发现FOXB2低表达与患者预后不良相关，抗体可用于免疫组化检测临床样本中的蛋白定位。
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3. **文献名称**: *"FOXB1/2 Antibody Cross-Reactivity Analysis in Murine and Human Tissues"*
**作者**: Garcia R, et al.
**摘要**: 通过比较不同物种(小鼠、人)中FOXB1和FOXB2的抗原表位，评估了商业抗体的交叉反应性。研究发现部分抗体存在非特异性结合，需结合基因敲除模型验证结果可靠性。
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4. **文献名称**: *"Role of FOXB1 in Lung Development: Insights from Antibody-Based Knockdown"*
**作者**: Chen L, et al.
**摘要**: 利用FOXB1抗体进行功能阻断实验，发现其在小鼠肺分支形态发生中起关键作用。抗体介导的蛋白抑制导致肺泡发育异常，提示FOXB1为潜在治疗靶点。
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**建议**：实际文献请通过PubMed、Google Scholar或Web of Science搜索关键词“FOXB1 antibody”或“FOXB2 antibody”，筛选涉及抗体开发、验证或应用的实验研究。
FOXB1 and FOXB2 (Forkhead Box B1/B2) are transcription factors belonging to the Forkhead box (Fox) protein family, characterized by a conserved forkhead DNA-binding domain. These proteins play critical roles in embryonic development, tissue differentiation, and cellular regulation. FOXB1 is notably involved in the development of the central nervous system and metabolic processes, while FOXB2 has been linked to organogenesis, particularly in the kidney and reproductive systems. Both proteins regulate gene expression by binding to specific DNA sequences, influencing cell fate and function.
Antibodies targeting FOXB1/2 are essential tools for studying their expression, localization, and molecular interactions. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to map protein distribution in tissues or cultured cells. Such antibodies help elucidate their roles in developmental pathways, disease mechanisms (e.g., cancer, metabolic disorders), and potential therapeutic targets. Commercial FOXB1/2 antibodies are typically raised in hosts like rabbits or mice, using immunogenic peptide sequences specific to conserved regions of the proteins.
Validation of antibody specificity is crucial, as cross-reactivity with other Fox family members can occur. Researchers often employ knockout controls or siRNA-mediated knockdown to confirm target specificity. These antibodies have advanced studies in developmental biology, neurobiology, and oncology, offering insights into gene regulatory networks and disease biomarkers.
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