| WB | 1/500-1/3000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Myocardin-related transcription factor A; MRTF-A; Megakaryoblastic leukemia 1 protein; Megakaryocytic acute leukemia protein; |
| Entrez GeneID | 57591; |
| WB Predicted band size | 99kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from N-terminal of human MKL1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是模拟生成的关于MKL1抗体的参考文献示例(实际文献请通过学术数据库查询)：
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1. **文献名称**: "MKL1/SRF Signaling Axis Promotes Tumor Metastasis via Cytoskeletal Remodeling"
**作者**: Wang, X. et al.
**摘要**: 研究利用MKL1抗体进行Western Blot和免疫组化，发现MKL1通过激活SRF调控肌动蛋白重组，促进乳腺癌细胞迁移和转移。
2. **文献名称**: "Role of MKL1 in Cardiac Fibroblast Activation and Fibrosis"
**作者**: Smith, J.R. & Zhang, L.
**摘要**: 通过MKL1抗体阻断实验，证明MKL1在TGF-β诱导的心肌纤维化中起关键作用，为抗纤维化治疗提供潜在靶点。
3. **文献名称**: "Development and Validation of a High-Specificity MKL1 Monoclonal Antibody"
**作者**: Tanaka, K. et al.
**摘要**: 报道一种新型MKL1单克隆抗体的开发，验证其在流式细胞术、免疫荧光中的特异性，适用于低表达量样本检测。
4. **文献名称**: "MKL1 Expression Correlates with Poor Prognosis in Glioblastoma"
**作者**: Chen, H. et al.
**摘要**: 使用MKL1抗体分析胶质母细胞瘤患者组织，发现MKL1高表达与肿瘤侵袭性和患者生存期缩短显著相关。
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**注**：以上为模拟内容，真实文献建议通过PubMed、Google Scholar等平台以关键词“MKL1 antibody”、“MRTF-A”或“MKL1 function”检索。
The MKL1 (Megakaryoblastic Leukemia 1) antibody is a tool used to study the MKL1 protein, also known as MRTF-A (Myocardin-Related Transcription Factor A). MKL1 is a transcriptional coactivator that binds to serum response factor (SRF), regulating genes involved in cytoskeletal dynamics, cell proliferation, and differentiation. It is activated by Rho GTPase signaling, which triggers its nuclear translocation upon cytoskeletal reorganization. Structurally, MKL1 contains RPEL domains for actin binding and nuclear localization signals (NLS). Dysregulation of MKL1 is linked to cancer metastasis, fibrosis, and cardiovascular diseases.
Antibodies targeting MKL1 are essential for detecting its expression, subcellular localization, and interaction partners in research. They are used in techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to explore MKL1's role in SRF-mediated transcription and pathological processes. For example, studies employing MKL1 antibodies have revealed its involvement in epithelial-mesenchymal transition (EMT) in cancer and fibroblast activation in fibrotic disorders. Commercial MKL1 antibodies are typically validated for specificity across human, mouse, and rat samples, aiding comparative studies. Understanding MKL1's mechanisms through antibody-based assays contributes to potential therapeutic strategies targeting SRF/MKL1 pathways in disease.
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