Rabbit Polyclonal HistoneH2B Antibody

Histone H2B is one of the five major histone proteins (H2A, H2B, H3. H4. and H1) that play a critical role in organizing eukaryotic DNA into chromatin. As a core component of nucleosomes, H2B forms a dimer with H2A, contributing to the structural stability of chromatin and regulating DNA accessibility for transcription, replication, and repair. Post-translational modifications (PTMs) of H2B, such as ubiquitination, phosphorylation, and acetylation, are dynamically involved in epigenetic regulation, DNA damage response, and cell cycle progression. Antibodies targeting histone H2B are essential tools for studying these modifications and their biological roles. They are widely used in techniques like chromatin immunoprecipitation (ChIP), Western blotting, and immunofluorescence to investigate histone dynamics, gene expression patterns, and chromatin remodeling mechanisms. H2B-specific antibodies also help distinguish between canonical H2B and its variants (e.g., H2B.Z, H2B.E), which have specialized functions in development and disease. Dysregulation of H2B or its PTMs has been linked to cancers, neurodegenerative disorders, and autoimmune diseases, making these antibodies valuable in both basic research and clinical diagnostics. Validation of antibody specificity is crucial, as cross-reactivity with other histones or isoforms can lead to misinterpretation of experimental results.