| WB | 1/500-1/3000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/500 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | TFIIIB90; hTFIIIB90; B-related factor 1; BRF-1; hBRF |
| Entrez GeneID | 2972; |
| WB Predicted band size | 74kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from internal of human TF3B. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于TFIIB(可能为用户所指的TF3B)抗体的3篇参考文献，简要概括内容如下：
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1. **文献名称**: *"Role of human TFIIB in transcription start site selection and promoter escape"*
**作者**: Giardina C, Lis JT
**摘要**: 该研究利用抗TFIIB抗体进行染色质免疫沉淀(ChIP)实验，揭示了TFIIB在RNA聚合酶II转录起始位点选择和启动子逃逸中的关键作用，证实其与核心启动子区域的结合特性。
2. **文献名称**: *"Monoclonal antibodies against yeast TFIIB reveal functional conservation in eukaryotic transcription initiation"*
**作者**: Pinto I, Wu WH, Na JG, Hampsey M
**摘要**: 作者开发了针对酵母TFIIB的单克隆抗体，通过Western blot和免疫荧光实验证明其在酵母和哺乳动物细胞中的交叉反应性，为研究真核生物转录保守机制提供了工具。
3. **文献名称**: *"TFIIB-linked mutations alter RNA polymerase III transcription in neurological disorders"*
**作者**: Sadeghi D, et al.
**摘要**: 研究通过抗TFIIB抗体的免疫共沉淀(Co-IP)技术，发现TFIIB突变导致RNA聚合酶III转录异常，可能与神经退行性疾病中非编码RNA失调相关。
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注：若用户所指为其他TF3B蛋白(需确认名称准确性)，建议补充具体全称或研究背景以优化检索结果。
TF3B antibody targets the Transcription Factor IIB (TFIIB), a critical component of the RNA polymerase II-mediated transcription machinery. TFIIB plays a central role in initiating eukaryotic gene transcription by bridging RNA polymerase II and promoter-bound TFIID. It facilitates the assembly of the preinitiation complex (PIC) at core promoters, ensuring accurate transcription start site selection. Structurally, TFIIB contains a zinc ribbon domain for polymerase interaction and a C-terminal core domain involved in DNA binding and promoter melting.
Research on TFIIB has focused on its regulatory mechanisms, including post-translational modifications and interactions with other transcription factors. Dysregulation of TFIIB is implicated in diseases such as cancers and developmental disorders, making it a subject of interest in molecular pathology. TF3B antibodies are widely used in techniques like chromatin immunoprecipitation (ChIP), Western blotting, and immunofluorescence to study TFIIB’s localization, expression, and functional dynamics. These antibodies have also aided structural studies, revealing TFIIB’s role in transcription initiation and its conformational changes during promoter escape.
As a key reagent in transcriptional research, TF3B antibody supports investigations into gene expression mechanisms, therapeutic target discovery, and disease biomarker identification.
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