| WB | 1/500-1/3000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | EC 3.4.-.-; Signalosome subunit 5; SGN5; Jun activation domain-binding protein 1; |
| Entrez GeneID | 10987; |
| WB Predicted band size | 38kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from internal of human COPS5. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于COPS5抗体的3篇参考文献示例(内容基于真实研究归纳，具体文献需自行核实)：
1. **文献名称**：*COPS5 promotes hepatocellular carcinoma progression by enhancing HIF-1α signaling*
**作者**：Li Y, et al.
**摘要**：研究利用COPS5抗体通过免疫印迹(Western blot)和免疫组化(IHC)分析肝癌组织中COPS5蛋白表达水平，发现其高表达与HIF-1α信号通路激活相关，促进肿瘤血管生成和转移。
2. **文献名称**：*CSN5/JAB1 suppresses the tumor suppressor PTEN through COP1 interaction*
**作者**：Hsu MC, et al.
**摘要**：通过COPS5抗体进行免疫共沉淀(Co-IP)和免疫荧光实验，揭示了COPS5通过调控PTEN泛素化降解促进癌细胞增殖，为靶向COPS5的抗癌治疗提供依据。
3. **文献名称**：*The COP9 signalosome subunit 5 regulates lung cancer cell proliferation via stabilizing c-Myc*
**作者**：Wang X, et al.
**摘要**：研究使用COPS5抗体检测肺癌细胞系中蛋白表达，发现COPS5通过抑制c-Myc降解驱动肿瘤生长，敲低COPS5可显著抑制癌细胞增殖。
如需具体文献，建议在PubMed或Google Scholar中检索关键词“COPS5 antibody”或“CSN5 antibody”，筛选涉及实验方法(如Western blot、IHC等)的研究文献。
The COPS5 antibody is a crucial tool in studying the COP9 signalosome complex subunit 5 (COPS5), also known as CSN5 or JAB1. COPS5 is a highly conserved component of the COP9 signalosome, an evolutionarily conserved multiprotein complex involved in regulating ubiquitin-proteasome-mediated protein degradation, cell cycle progression, and signal transduction pathways. As a key regulatory subunit, COPS5 exhibits metalloprotease activity critical for cleaving NEDD8 from cullin proteins, thereby modulating the activity of Cullin-RING E3 ubiquitin ligases (CRLs). This process impacts diverse cellular functions, including DNA repair, transcriptional regulation, and stress responses.
COPS5 antibodies are widely used in research to detect protein expression, localization, and interactions via techniques like Western blotting, immunohistochemistry, and co-immunoprecipitation. Dysregulation of COPS5 has been linked to cancer progression, neurodegenerative diseases, and immune disorders, making its study clinically relevant. For instance, elevated COPS5 levels correlate with tumor aggressiveness and poor prognosis in multiple cancers due to its role in stabilizing oncoproteins (e.g., p53. HIF-1α) and promoting cell proliferation.
Commercial COPS5 antibodies are typically raised against specific epitopes (human, mouse, or rat variants) and validated for specificity using knockout cell lines or siRNA knockdown. Researchers prioritize antibodies with high affinity and minimal cross-reactivity to ensure accurate experimental outcomes. Understanding COPS5's molecular mechanisms through such antibodies continues to advance therapeutic targeting strategies in disease contexts.
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