Rabbit Polyclonal 45170 Antibody

The 45170 antibody, often referenced by its product identifier (e.g., catalog # PA5-45170 from Thermo Fisher Scientific), is a rabbit polyclonal antibody designed to detect phosphorylated CCAAT/enhancer-binding protein beta (C/EBPβ) at threonine 235 (Thr235). C/EBPβ is a transcription factor involved in critical cellular processes, including differentiation, inflammation, and metabolism. Phosphorylation at Thr235 is associated with its activation, particularly in response to signaling pathways like MAPK, which regulate immune responses and stress adaptation. This antibody is widely used in techniques such as Western blotting (WB), immunohistochemistry (IHC), and ELISA to study C/EBPβ's role in diseases like cancer, metabolic disorders, and chronic inflammation. Researchers employ it to explore mechanisms underlying conditions such as liver fibrosis, adipose tissue dysfunction, and tumor progression, where dysregulated C/EBPβ activity is implicated. By specifically targeting the phosphorylated form, the 45170 antibody helps elucidate post-translational regulatory mechanisms, offering insights into therapeutic targets. Its validation in multiple applications and species underscores its utility in both basic and translational research contexts.