| WB | 1/500-1/3000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Docking protein 4; Downstream of tyrosine kinase 4; DOK4; |
| Entrez GeneID | 55715; |
| WB Predicted band size | 37kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from internal of human DOK4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于DOK4抗体的相关文献概览：
1. **"DOK4 regulates neutrophil migration and adhesion in inflammatory responses"**
- **作者**: Lee Y et al.
- **摘要**: 本研究利用特异性DOK4抗体，通过免疫印迹和免疫荧光技术，发现DOK4通过调控Rho GTPases活性影响中性粒细胞的迁移和黏附，揭示了其在炎症反应中的关键作用。
2. **"Downstream of tyrosine kinase 4 (DOK4) as a potential tumor suppressor in colorectal cancer"**
- **作者**: Chen H et al.
- **摘要**: 通过DOK4抗体检测结直肠癌组织样本，发现DOK4表达显著降低，且与Wnt/β-catenin信号通路抑制相关，提示其可能通过抑制致癌通路发挥抑癌功能。
3. **"Characterization of DOK4 antibody specificity in hematopoietic malignancies"**
- **作者**: Müller R et al.
- **摘要**: 文章验证了多种商业DOK4抗体的特异性，发现部分抗体存在交叉反应性，并筛选出适用于流式细胞术和免疫沉淀的高效抗体，为血液肿瘤研究提供工具支持。
注：以上文献为示例性内容，实际引用需根据具体研究补充真实文献信息。建议通过PubMed或Web of Science以"DOK4 antibody"为关键词检索最新研究。
The DOK4 (Docking Protein 4) antibody is a research tool used to study the DOK4 protein, a member of the DOK family of adaptor proteins involved in signal transduction. DOK proteins act as scaffolding molecules, regulating tyrosine kinase-mediated signaling pathways. Specifically, DOK4 interacts with receptor tyrosine kinases (e.g., RET, insulin receptor) and downstream effectors to modulate cellular processes such as proliferation, differentiation, and apoptosis. It contains conserved domains, including an N-terminal pleckstrin homology (PH) domain and phosphotyrosine-binding (PTB) domain, which facilitate protein-protein interactions and membrane localization.
DOK4 is implicated in various physiological and pathological contexts. Studies highlight its role in neuronal development, immune regulation, and cancer. For instance, DOK4 overexpression has been linked to certain malignancies, where it may promote survival signals via Ras/MAPK or PI3K/AKT pathways. Conversely, reduced DOK4 expression is associated with metabolic disorders like diabetes. The DOK4 antibody enables detection and quantification of DOK4 in tissues or cell lines, aiding investigations into its expression patterns, post-translational modifications, and mechanistic contributions to diseases. It is commonly used in techniques like Western blotting, immunohistochemistry, and immunofluorescence. Research utilizing this antibody continues to clarify DOK4's dual roles as a tumor suppressor or oncogene, depending on cellular context, underscoring its therapeutic potential.
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