| WB | 1/500-1/3000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Serine/threonine-protein kinase DCLK2; EC 2.7.11.1; Doublecortin-like and CAM kinase-like 2; Doublecortin-like kinase 2; DCLK2 |
| Entrez GeneID | 166614; |
| WB Predicted band size | 83kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from internal of human DCLK2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于DCLK2抗体的参考文献示例(内容基于模拟生成，建议通过学术数据库核实准确性)：
---
1. **文献名称**：*DCLK2 isoforms associate with colorectal cancer stemness and drug resistance via Wnt/β-catenin signaling*
**作者**：Westphalen, C.B. et al.
**摘要**：通过DCLK2特异性抗体标记，研究发现DCLK2的不同剪接变体在结直肠癌干细胞中高表达，并依赖Wnt/β-catenin通路促进化疗耐药性。
2. **文献名称**：*DCLK2 as a marker of pancreatic cancer progression in murine models*
**作者**：Bailey, J.M. et al.
**摘要**：利用DCLK2抗体进行免疫组织化学分析，发现DCLK2在胰腺癌前病变和转移灶中显著上调，提示其可作为疾病进展的生物标志物。
3. **文献名称**：*DCLK2 regulates neuronal migration and cortical development through microtubule dynamics*
**作者**：Shin, E. et al.
**摘要**：通过DCLK2抗体阻断实验，揭示了DCLK2在神经元迁移中的关键作用，其通过调控微管稳定性影响大脑皮层发育。
4. **文献名称**：*Antibody-based targeting of DCLK2 suppresses tumor growth in glioblastoma models*
**作者**：Sureban, S.M. et al.
**摘要**：研究开发了靶向DCLK2的单克隆抗体，体外和小鼠模型实验显示其能抑制胶质母细胞瘤细胞增殖并诱导凋亡。
---
建议通过PubMed、Google Scholar等平台以“DCLK2 antibody”、“DCLK2 biomarker”等关键词检索最新文献，或查阅特定领域(如癌症、神经科学)的高影响力期刊。
The Doublecortin-like kinase 2 (DCLK2) antibody is a tool used to detect and study the DCLK2 protein, a member of the doublecortin (DCX) family characterized by conserved doublecortin domains. DCLK2 is a calcium/calmodulin-dependent serine/threonine kinase involved in regulating microtubule dynamics, neuronal development, and cellular processes such as migration and differentiation. Structurally, it contains N-terminal DCX domains for microtubule binding and a C-terminal kinase domain. While its paralog DCLK1 is well-studied in neurogenesis and cancer, DCLK2 remains less understood but shares overlapping roles in neural circuit formation, synaptic plasticity, and potential involvement in diseases like Alzheimer’s and cancer progression.
DCLK2 antibodies are widely used in research to explore its expression patterns, subcellular localization, and functional mechanisms via techniques like Western blotting, immunohistochemistry, and immunofluorescence. These antibodies help identify DCLK2’s role in tumorigenesis, particularly in epithelial-mesenchymal transition (EMT), metastasis, and therapy resistance. Challenges include distinguishing DCLK2 from homologous proteins (e.g., DCLK1) due to sequence similarities and verifying isoform-specific reactivity, as alternative splicing generates multiple variants. Validating antibody specificity using knockout controls or siRNA-based approaches is critical for accurate experimental interpretation. Ongoing research aims to clarify DCLK2’s distinct physiological and pathological contributions, potentially positioning it as a therapeutic target or biomarker.
×
