| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | CA8 |
| Uniprot No | P35219 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-290aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSHMADLSF IEDTVAFPEK EEDEEEEEEG VEWGYEEGVE WGLVFPDANG EYQSPINLNS REARYDPSLL DVRLSPNYVV CRDCEVTNDG HTIQVILKSK SVLSGGPLPQ GHEFELYEVR FHWGRENQRG SEHTVNFKAF PMELHLIHWN STLFGSIDEA VGKPHGIAII ALFVQIGKEH VGLKAVTEIL QDIQYKGKSK TIPCFNPNTL LPDPLLRDYW VYEGSLTIPP CSEGVTWILF RYPLTISQLQ IEEFRRLRTH VKGAELVEGC DGILGDNFRP TQPLSDRVIR AAFQ |
| 预测分子量 | 36 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CA8重组蛋白的3篇参考文献及简要摘要：
1. **文献名称**：*"Carbonic Anhydrase VIII (CA8) Modulates Inositol 1.4.5-Trisphosphate Receptor Function via Direct Interaction"*
**作者**：Hirota J. et al.
**摘要**：研究发现重组CA8蛋白通过与肌醇三磷酸受体(IP3R)直接结合，调控钙离子信号传导，揭示了其在神经细胞钙稳态中的作用机制。
2. **文献名称**：*"Expression and Purification of Recombinant CA8 Protein for Structural Studies"*
**作者**：Smith K.L. et al.
**摘要**：描述了CA8重组蛋白在大肠杆菌系统中的高效表达与纯化方法，并通过X射线晶体学分析其三维结构，为功能研究提供基础。
3. **文献名称**：*"Role of CA8 in Cerebellar Ataxia: Insights from Recombinant Protein Rescue in a Mouse Model"*
**作者**：Chen R. et al.
**摘要**：利用重组CA8蛋白在小鼠模型中恢复CA8缺陷导致的运动失调表型，证实CA8在维持小脑功能中的关键作用。
4. **文献名称**：*"CA8 as a Biomarker in Neurodegenerative Diseases: Recombinant Protein-Based Immunoassays"*
**作者**：Garcia M. et al.
**摘要**：开发了基于重组CA8蛋白的ELISA检测方法，探讨了其在脊髓小脑共济失调等疾病中的潜在诊断价值。
(注：以上文献信息为示例性质，实际引用需核对真实数据库及原文。)
**Background of CA8 Recombinant Protein**
Carbonic anhydrase VIII (CA8), a member of the carbonic anhydrase (CA) family, is a unique isoform distinguished by its lack of enzymatic activity due to the absence of critical histidine residues required for catalytic function. Unlike other CAs, which regulate pH and CO₂ metabolism, CA8 is thought to act as a regulatory or structural protein. It is primarily expressed in the cerebellum and has been implicated in neuronal development, synaptic signaling, and calcium homeostasis.
The CA8 gene, located on chromosome 8 in humans, encodes a 28 kDa protein that shares homology with other CA family members but retains distinct structural features. Studies link CA8 mutations or dysregulation to neurological disorders such as cerebellar ataxia, autism spectrum disorders, and intellectual disabilities, highlighting its role in maintaining cerebellar integrity.
Recombinant CA8 protein is produced via genetic engineering, typically using bacterial (e.g., *E. coli*) or mammalian expression systems. This involves cloning the CA8 gene into an expression vector, followed by transfection, protein purification (e.g., affinity chromatography), and validation (e.g., Western blot, mass spectrometry). Recombinant CA8 enables functional studies, including its interaction with inositol 1.4.5-trisphosphate receptors (IP3Rs) in calcium signaling pathways, and its potential as a therapeutic target or biomarker.
Research on recombinant CA8 has advanced understanding of its non-enzymatic roles in neurobiology and disease mechanisms, offering insights for diagnostic or therapeutic strategies in cerebellar pathologies. Its production continues to support structural analyses and drug discovery efforts targeting CA8-associated disorders.
×
