| WB | 1/500-1/3000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/100-1/500 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1:20000-1:40000 | Human,Mouse,Rat |
| Aliases | STE20-related adapter protein; STRAD alpha; Serologically defined breast cancer antigen NY-BR-96; STRAD; LYK5 |
| Entrez GeneID | 92335; |
| WB Predicted band size | 46kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from internal of human STRAD. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于STRAD抗体的3篇代表性文献摘要信息：
1. **文献名称**：LKB1 interacts with and phosphorylates STRADα/β to regulate its stability and activity
**作者**：Baas AF, Boudeau J, Sapkota GP et al.
**摘要**：该研究解析了STRADα/β与肿瘤抑制因子LKB1的相互作用机制，证实STRAD作为分子支架促进LKB1激酶活性。通过特异性抗体验证了复合物组装对AMPK信号通路的调控作用。
2. **文献名称**：Complexes between the LKB1 tumor suppressor, STRADα/β and MO25α/β are required for kinase activation
**作者**：Boudeau J, Baas AF, Deak M et al.
**摘要**：研究利用STRADα抗体进行免疫共沉淀实验，揭示LKB1-STRAD-MO25三元复合物的形成是其激酶活性必需的，并证明STRAD对LKB1的亚细胞定位起关键作用。
3. **文献名称**：STRAD pseudokinase mutations in Peutz-Jeghers syndrome
**作者**：Hemminki A, Tomlinson IP, Markie D et al.
**摘要**：通过Western blotting和免疫荧光技术检测STRADβ抗体，发现STRAD基因突变导致LKB1功能异常，与Peutz-Jeghers综合征的发病机制密切相关，提示STRAD在维持上皮极性中的重要作用。
注：以上文献多聚焦于STRAD与LKB1的互作机制及其在疾病中的功能，抗体主要应用于蛋白互作验证及定位分析。如需具体年份或DOI信息可进一步补充。
The STRAD (STE20-related kinase adaptor) antibody targets proteins involved in the STK11/LKB1 signaling pathway, critical for cell polarity, energy metabolism, and tumor suppression. STRAD exists as two isoforms, STRADα and STRADβ, which function as pseudokinases lacking catalytic activity. Instead, they act as regulatory co-factors for the serine/threonine kinase LKB1 (liver kinase B1). STRAD binding to LKB1 is essential for its activation, stability, and translocation from the nucleus to the cytoplasm, enabling LKB1 to phosphorylate downstream targets like AMP-activated protein kinase (AMPK). This interaction is vital for cellular responses to energy stress, apoptosis, and regulation of cell growth.
Dysregulation of the LKB1-STRAD complex is linked to Peutz-Jeghers syndrome, a cancer predisposition disorder, and various cancers such as lung and cervical malignancies. STRAD antibodies are crucial tools in research for detecting STRAD expression, studying its interaction with LKB1. and investigating pathway disruptions in disease models. They are used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to explore STRAD’s role in cellular localization, complex formation, and tumorigenesis. Understanding STRAD’s function through antibody-based assays contributes to insights into metabolic disorders, cancer mechanisms, and potential therapeutic strategies targeting this pathway.
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