| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Mitogen-activated protein kinase kinase kinase; EC 2.7.11.25; Mixed lineage kinase 4; MLK4; KIAA1804 |
| Entrez GeneID | 84451; |
| WB Predicted band size | 110kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from internal of human MAP3KL4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MAP3KL4(MEKK4)抗体的3篇示例参考文献(注：以下内容为模拟示例，实际文献需通过学术数据库验证)：
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1. **文献名称**: *MEKK4 regulates embryonic vascular development through the Notch signaling pathway*
**作者**: Li, X., et al.
**摘要**: 本研究利用MAP3KL4特异性抗体进行免疫组化和Western blot分析，发现MEKK4通过调控Notch信号通路影响小鼠胚胎血管形成。抗体验证显示MEKK4在血管内皮细胞中高表达，基因敲除导致血管发育异常。
2. **文献名称**: *Role of MAP3KL4 in TGF-β-induced apoptosis in cancer cells*
**作者**: Chen, Y., et al.
**摘要**: 通过MAP3KL4抗体进行免疫沉淀和激酶活性检测，研究发现MEKK4在TGF-β介导的肿瘤细胞凋亡中起关键作用。抗体特异性实验证实其在结直肠癌细胞系中的表达水平与化疗敏感性相关。
3. **文献名称**: *MEKK4 modulates oxidative stress response in neuronal cells*
**作者**: Park, J., & Kim, S.
**摘要**: 使用MAP3KL4抗体进行免疫荧光和流式细胞术，发现MEKK4通过激活p38 MAPK通路参与神经元细胞的氧化应激应答。抗体阻断实验表明其活性缺失会加剧ROS诱导的细胞死亡。
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**注意事项**：
- 以上文献为示例，实际引用需查询PubMed、Web of Science等平台(可搜索关键词：MAP3KL4 antibody、MEKK4 Western blot/immunoprecipitation等)。
- 实验应用中，建议关注抗体来源(如商用抗体的品牌、货号)及验证方法(如敲除/敲低细胞系的阴性对照)。
The MAP3KL4 antibody is a research tool designed to detect mitogen-activated protein kinase kinase kinase-like 4 (MAP3KL4), also known as MEKK4 or MAP3K21. MAP3KL4 is a serine/threonine kinase within the MAPK signaling cascade, a critical pathway regulating cellular responses to stress, proliferation, differentiation, and apoptosis. It functions upstream of MAPK pathways, including p38 and JNK, and is implicated in diverse processes such as embryonic development, immune regulation, and tissue homeostasis. Dysregulation of MAP3KL4 has been linked to pathologies like cancer, inflammatory disorders, and neurological diseases.
The antibody is typically produced in hosts such as rabbits or mice using immunogenic peptides or recombinant proteins specific to MAP3KL4. It enables researchers to study the protein's expression, localization, and post-translational modifications via techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and immunoprecipitation (IP). Validation often includes testing in knockout cell lines or tissues to confirm specificity.
MAP3KL4 antibodies are vital for elucidating the kinase's role in signaling networks, its interaction partners, and its context-dependent activation mechanisms. Such studies contribute to understanding disease mechanisms and identifying potential therapeutic targets. Researchers must optimize experimental conditions (e.g., dilution ratios, blocking buffers) based on sample type and application to ensure reliable results.
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