| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Shugoshin-like 1; hSgo1; Serologically defined breast cancer antigen NY-BR-85; SGO1; |
| Entrez GeneID | 151648; |
| WB Predicted band size | 64kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from internal of human SGOL1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于SGOL1抗体的3篇参考文献及其简要摘要：
1. **文献名称**: *Shugoshin collaborates with protein phosphatase 2A to protect cohesin*
**作者**: Kitajima TS, et al.
**摘要**: 该研究通过使用SGOL1抗体进行免疫沉淀和染色质分析，揭示了SGOL1在保护姐妹染色单体黏连蛋白复合体中的作用，并阐明其与蛋白磷酸酶2A(PP2A)的协同机制，确保有丝分裂中染色体的正确分离。
2. **文献名称**: *SGOL1 expression is a prognostic marker in multiple cancer types*
**作者**: Zhang L, et al.
**摘要**: 研究利用SGOL1抗体对多种癌症组织进行免疫组化分析，发现SGOL1的高表达与肿瘤患者的不良预后相关，提示其可能通过调控染色体稳定性促进肿瘤进展。
3. **文献名称**: *Development and validation of a novel SGOL1 monoclonal antibody for functional studies*
**作者**: Tanaka Y, et al.
**摘要**: 该文献报道了一种高特异性SGOL1单克隆抗体的开发，并通过Western blot、免疫荧光等技术验证其应用，证明该抗体能有效检测SGOL1在细胞周期不同阶段的定位变化。
这些研究涵盖了SGOL1的功能机制、临床意义及实验工具开发，可作为相关研究的参考。
The Shugoshin-like 1 (SGOL1) protein is a conserved member of the shugoshin family, playing a critical role in preserving genomic stability during cell division. It is primarily involved in protecting centromeric cohesion between sister chromatids during mitosis and meiosis, ensuring accurate chromosome segregation. SGOL1 achieves this by recruiting protein phosphatase 2A (PP2A) to centromeres, which counteracts phosphorylation of cohesin complexes, thereby preventing premature separation. Dysregulation of SGOL1 has been linked to chromosomal instability, a hallmark of many cancers and developmental disorders.
SGOL1 antibodies are essential tools for studying its localization, expression, and functional mechanisms in cellular models. These antibodies are widely used in techniques like immunofluorescence, Western blotting, and immunohistochemistry to visualize SGOL1's dynamic centromeric localization during different cell cycle phases. Research utilizing SGOL1 antibodies has highlighted its dual isoforms (SGOL1A and SGOL1B) with distinct roles—SGOL1A in mitosis and SGOL1B in meiosis. Additionally, studies explore SGOL1's potential as a therapeutic target, given its overexpression in certain cancers and association with poor prognosis. However, challenges remain in understanding isoform-specific interactions and context-dependent regulatory networks.
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