Rabbit Polyclonal PFKFB1/4 Antibody

The PFKFB (6-phosphofructo-2-kinase/fructose-2.6-bisphosphatase) family comprises bifunctional enzymes that regulate cellular fructose-2.6-bisphosphate (F2.6-BP) levels, a key allosteric activator of phosphofructokinase-1 (PFK-1) in glycolysis. PFKFB1 (also called PFK-2/FBPase-2) and PFKFB4 are two isoforms with distinct tissue expression and regulatory roles. PFKFB1 is ubiquitously expressed and functions as an inducible isoform, sensitive to hormonal signals like insulin. It primarily promotes glycolytic flux by synthesizing F2.6-BP. PFKFB4. often termed the "cancer-related isoform," is highly expressed in steroidogenic tissues (e.g., testes, prostate) and cancers. It exhibits stronger kinase activity, favoring glycolytic metabolism under hypoxic or stress conditions, and is regulated by hypoxia-inducible factors (HIF-1α) and oncogenic pathways. Both isoforms also possess bisphosphatase activity, but their net effect typically leans toward F2.6-BP production. Antibodies targeting PFKFB1/4 are crucial tools for studying metabolic reprogramming in diseases, particularly cancer. They enable detection of protein expression, localization, and post-translational modifications via techniques like Western blot, immunohistochemistry, and immunofluorescence. Dysregulation of PFKFB1/4 is linked to pathologies including metabolic syndrome, neurodegeneration, and tumor progression, making these antibodies valuable for mechanistic and therapeutic research. Specificity validation (e.g., knockout controls) is essential due to isoform homology.