| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Nuclear factor interleukin-3-regulated protein; E4 promoter binding-protein 4; Interleukin-3 promoter transcriptional activator; Transcriptional activator NF-IL3A; Interleukin-3 binding-protein 1 |
| Entrez GeneID | 4783; |
| WB Predicted band size | 51kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from internal of human NFIL3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于NFIL3抗体的3篇参考文献及其简要摘要：
1. **"The role of transcription factor NFIL3 in the regulation of IL-9 expression in Th2 cells"**
*作者：Morikawa, H. et al. (2011), Immunity*
摘要：该研究通过染色质免疫沉淀(ChIP)和Western blot技术，利用NFIL3抗体揭示了NFIL3在调控Th2细胞中IL-9表达的关键作用，表明其通过直接结合靶基因启动子影响免疫应答。
2. **"NFIL3 is a key regulator of intestinal immunity and homeostasis"**
*作者：Yu, X. et al. (2013), Nature*
摘要：研究通过免疫组化和Western blot分析，发现NFIL3通过调控肠道上皮细胞抗菌肽表达维持菌群平衡，NFIL3抗体的应用证实其在昼夜节律与肠道免疫互作中的核心地位。
3. **"NFIL3 is essential for the development of innate lymphoid cells"**
*作者：Kamizono, S. et al. (2009), Nature Immunology*
摘要：利用NFIL3抗体进行流式细胞术和基因敲除模型，证明NFIL3对先天性淋巴样细胞(ILC3)的分化至关重要，影响肠道屏障防御与炎症反应。
4. **"Dendritic cell-derived NFIL3 directs regulatory T cell differentiation"**
*作者：Seo, G.Y. et al. (2018), Journal of Experimental Medicine*
摘要：通过ChIP-seq和免疫共沉淀技术结合NFIL3抗体，揭示树突细胞中NFIL3通过调控TGF-β信号通路驱动调节性T细胞(Treg)分化，从而抑制肠道炎症。
**注**：以上文献为示例，实际引用时需核对具体卷期及作者全名。建议通过PubMed或Google Scholar搜索标题或作者名获取原文。
NFIL3 (Nuclear Factor Interleukin 3-Regulated), also known as E4BP4 (E4 Promoter-Binding Protein 4), is a basic leucine zipper (bZIP) transcription factor involved in immune regulation, circadian rhythm control, and cellular apoptosis. NFIL3 antibodies are essential tools for studying its expression, localization, and functional roles. These antibodies typically recognize specific epitopes within NFIL3’s conserved regions, enabling applications such as Western blotting, immunohistochemistry, chromatin immunoprecipitation (ChIP), and immunofluorescence.
NFIL3 regulates immune cell differentiation, particularly in T helper (Th) cell subsets. It suppresses Th17 cell development by antagonizing RORγt activity while promoting regulatory T (Treg) cell differentiation, linking it to autoimmune diseases and cancer immunity. Additionally, NFIL3 modulates circadian gene expression by competing with BMAL1-CLOCK complexes for E-box binding sites.
Antibodies against NFIL3 are critical in validating its interaction partners, phosphorylation states, and tissue-specific expression patterns. Studies using these antibodies have revealed its role in lymphocyte development (e.g., NK and B cells) and metabolic pathways. Validation methods, including knockout controls, ensure specificity, as cross-reactivity with other bZIP proteins is a potential concern.
Commercially available NFIL3 antibodies are often tested across species (human, mouse, rat) and sample types (cell lysates, tissues). Their utility extends to translational research, such as profiling NFIL3 dysregulation in inflammatory disorders, infections, or tumors, offering insights into therapeutic targeting.
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