| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | TATA box-binding protein-associated factor 1A; TBP-associated factor 1A; TBP-associated factor RNA polymerase I 48 kDa; TAFI48; Transcription factor SL1 |
| Entrez GeneID | 9015; |
| WB Predicted band size | 53kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | Synthesized peptide derived from internal of human TAF1A. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于TAF1A抗体的3篇参考文献示例(注：以下内容为模拟生成，非真实文献)：
1. **文献名称**：**"TAF1A regulates ribosomal RNA transcription through interaction with RNA polymerase I"**
**作者**：Chen L, et al.
**摘要**：本研究利用TAF1A特异性抗体进行染色质免疫沉淀(ChIP)和免疫荧光实验，揭示TAF1A通过与RNA聚合酶I结合，调控核糖体RNA的转录起始，并影响细胞增殖。
2. **文献名称**：**"Development of a monoclonal antibody against human TAF1A for cancer biomarker screening"**
**作者**：Sato M, et al.
**摘要**：报道了一种高特异性TAF1A单克隆抗体的制备，并通过免疫组化验证其在结直肠癌组织中异常高表达，提示TAF1A可能作为潜在的肿瘤标志物。
3. **文献名称**：**"TAF1A interacts with the Sin3/HDAC complex to modulate gene silencing"**
**作者**：Gupta S, et al.
**摘要**：通过TAF1A抗体的免疫共沉淀(Co-IP)实验，发现TAF1A与Sin3/组蛋白去乙酰化酶复合物相互作用，参与表观遗传调控和靶基因的转录抑制。
4. **文献名称**：**"TAF1A knockout mice exhibit embryonic lethality and neural tube defects"**
**作者**：Wang Y, et al.
**摘要**：利用TAF1A抗体进行蛋白表达分析，发现TAF1A基因敲除导致小鼠胚胎早期死亡及神经管发育异常，表明其在胚胎发育中的关键作用。
(注：实际文献需通过PubMed、Google Scholar等平台检索确认。)
TAF1A (TATA box-binding protein-associated factor 1A) is a subunit of the selectivity factor SL1 complex, which is essential for RNA polymerase I (Pol I)-mediated transcription of ribosomal RNA (rRNA) genes. As a core component of the Pol I transcription machinery, TAF1A plays a critical role in ribosome biogenesis by facilitating the assembly of the pre-initiation complex at rRNA gene promoters. It interacts with other SL1 subunits (TAF1B, TAF1C, and TBP) and anchors the complex to the ribosomal DNA promoter, ensuring accurate transcription initiation. Dysregulation of TAF1A is linked to aberrant cell proliferation, cancer progression, and developmental disorders due to its central role in controlling cellular growth and protein synthesis.
TAF1A antibodies are widely used in research to investigate rRNA transcription mechanisms, nucleolar organization, and cellular stress responses. These antibodies enable the detection and localization of TAF1A in various experimental applications, including Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP). Studies utilizing TAF1A antibodies have revealed its overexpression in certain cancers, highlighting its potential as a therapeutic target. Additionally, they aid in exploring crosstalk between Pol I activity and signaling pathways like mTOR and p53. Validation of TAF1A antibodies is crucial, as specificity ensures accurate interpretation of its regulatory roles in health and disease.
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