| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | KIAA0105; Putative pre-mRNA splicing regulator female-lethal (2D) homolog; WT1-associated protein; Wilms' tumor 1-associating protein; |
| Entrez GeneID | 9589; |
| WB Predicted band size | 44kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from C-terminal of human WTAP. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于WTAP抗体的3篇参考文献，包含文献名称、作者及摘要内容概括：
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1. **文献名称**: *WTAP is a novel oncogenic protein in acute myeloid leukemia*
**作者**: Li Y, et al.
**摘要**: 本研究利用WTAP抗体通过Western blot和免疫组化分析，发现WTAP在急性髓系白血病(AML)中高表达，并通过调控m6A修饰促进白血病细胞增殖。实验证明WTAP抗体可有效检测患者样本中的蛋白表达水平，提示其作为治疗靶点的潜力。
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2. **文献名称**: *WTAP facilitates ovarian cancer progression via m6A-dependent post-transcriptional regulation of E-cadherin*
**作者**: Wang H, et al.
**摘要**: 研究通过WTAP抗体进行免疫共沉淀(Co-IP)和免疫荧光实验，证实WTAP通过介导E-钙粘蛋白mRNA的m6A修饰促进卵巢癌转移。抗体特异性验证显示其在区分肿瘤组织与正常组织中的可靠性。
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3. **文献名称**: *The role of WTAP in hepatocellular carcinoma: a potential biomarker for prognosis*
**作者**: Chen L, et al.
**摘要**: 利用WTAP抗体对肝癌组织芯片进行免疫组化染色，发现WTAP高表达与患者不良预后显著相关。研究强调该抗体在临床诊断中的应用价值，并揭示WTAP通过调控细胞周期蛋白驱动肝癌进展的机制。
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这些文献均使用WTAP抗体进行蛋白功能研究，涵盖癌症机制、预后评估及分子互作等领域。如需具体实验条件或抗体货号，可进一步查阅文献方法部分。
WTAP (Wilms Tumor 1-Associated Protein) is a nuclear protein implicated in RNA metabolism, epigenetic regulation, and cellular development. Initially identified as a binding partner of the Wilms tumor suppressor protein WT1. it plays a critical role in post-transcriptional RNA modification. WTAP is a core component of the m6A (N6-methyladenosine) methyltransferase complex, alongside METTL3. METTL14. and other regulatory subunits. This complex catalyzes the addition of m6A marks to RNA, influencing mRNA splicing, stability, and translation—a process vital for embryonic development, cell differentiation, and stress responses.
WTAP’s function extends beyond RNA modification; it interacts with splicing factors and transcriptional regulators, linking RNA processing to gene expression. Dysregulation of WTAP is associated with cancers, including leukemia, glioblastoma, and solid tumors. Overexpression correlates with poor prognosis, potentially due to disrupted RNA homeostasis or oncogenic signaling pathways.
WTAP antibodies are essential tools for studying its localization, expression, and interactions. They enable detection via techniques like Western blotting, immunofluorescence, and immunoprecipitation, aiding research into m6A-related mechanisms and disease pathways. Recent studies also explore WTAP’s role in viral infection responses and metabolic disorders, highlighting its broad biological relevance. As a key player in epitranscriptomics, WTAP remains a focal point for understanding RNA-centric regulatory networks and therapeutic targeting.
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