| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAB11B |
| Uniprot No | Q15907 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-215aa |
| 氨基酸序列 | GTRDDEYDYL FKVVLIGDSG VGKSNLLSRF TRNEFNLESK STIGVEFATR SIQVDGKTI KAQIWDTAGQ ERYRAITSAY YRGAVGALLV YDIAKHLTYE NVERWLKELR DHADSNIVIM LVGNKSDLR HLRAVPTDEA RAFAEKNNLS FIETSALDST NVEEAFKNIL TEIYRIVSQK QIADRAAHD ESPGNNVVDI SVPPTTDGQK PNKLQCC |
| 预测分子量 | 26 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RAB11B重组蛋白的3篇代表性文献摘要(作者和标题为虚构示例，仅供参考)：
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1. **标题**：*RAB11B regulates endosomal recycling through GTP-dependent membrane tethering*
**作者**：Schwartz SL, et al.
**摘要**：本研究利用重组RAB11B蛋白进行体外膜结合实验，揭示了其GTP酶活性对回收内体与质膜间囊泡运输的调控机制，证实RAB11B通过结合效应蛋白调控运输复合体组装。
2. **标题**：*Dynamic localization of RAB11B in polarized epithelial cells*
**作者**：Ng EL, et al.
**摘要**：通过表达GFP标记的重组RAB11B蛋白，发现其在细胞极性形成过程中富集于基底侧膜区，并参与细胞间紧密连接蛋白的循环运输，依赖其C端结构域定位。
3. **标题**：*RAB11B interacts with the exocyst complex to promote cytokinesis*
**作者**：Horgan CP, et al.
**摘要**：利用重组RAB11B进行免疫共沉淀实验，发现其直接结合Exo70亚基，调控分裂末期膜泡运输至中间体，敲低RAB11B导致胞质分裂缺陷。
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注：以上内容为模拟示例，实际文献需通过PubMed或Google Scholar检索关键词“RAB11B recombinant”或“RAB11B expression”获取。推荐使用真实数据库查询具体文献。
RAB11B recombinant protein is a key tool in studying intracellular trafficking mechanisms, particularly in vesicle-mediated transport and membrane dynamics. Belonging to the RAB GTPase family, RAB11B regulates the recycling of endosomes to the plasma membrane and trans-Golgi network, influencing processes like cytokinesis, autophagy, and receptor recycling. Its role in maintaining cellular homeostasis makes it a focus in cancer, neurodegenerative diseases, and developmental disorders research.
Recombinant RAB11B is typically produced using bacterial (e.g., E. coli) or mammalian expression systems, enabling high-purity protein production for structural and functional studies. The recombinant form often includes tags (e.g., His-tag) for simplified purification and tracking. Researchers use it to investigate GTP/GDP binding cycles, interactions with effector proteins (e.g., Rab11-FIPs), and regulatory mechanisms in membrane trafficking pathways.
Applications extend to drug discovery, where RAB11B activity modulation is explored for therapeutic interventions. Mutant variants (e.g., constitutively active Q70L or dominant-negative S25N) help dissect its GTPase-dependent functions. Studies also link RAB11B dysregulation to pathological conditions, including tumor metastasis and ciliopathies, highlighting its biomedical relevance. Proper post-translational modifications (e.g., prenylation) in recombinant versions ensure physiological relevance in in vitro assays.
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