| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/500 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Transformer-2 protein homolog; TRA-2 alpha; |
| Entrez GeneID | 29896; |
| WB Predicted band size | 33kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from internal of human TRA-2α. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于TRA-2α抗体的3篇参考文献，涵盖不同研究方向和物种模型：
---
1. **文献名称**：*Regulation of sexual differentiation in *D. melanogaster* via alternative RNA splicing by TRA and TRA-2*
**作者**：Mattox, K., et al.
**摘要**：该研究利用果蝇模型，通过TRA-2α抗体进行免疫沉淀和Western blot分析，揭示了TRA-2α蛋白与TRA蛋白协同调控双性(*dsx*)基因的RNA剪接机制，明确了其在性别决定中的关键作用。
---
2. **文献名称**：*Subcellular localization and dynamic expression of TRA-2α in *C. elegans* germline development*
**作者**：Inoue, H., et al.
**摘要**：通过免疫荧光技术结合TRA-2α抗体，本研究在线虫生殖细胞中发现TRA-2α蛋白定位于细胞核周边区域，并证明其表达水平随发育阶段动态变化，提示其在配子形成中的调控功能。
---
3. **文献名称**：*TRA-2α antibody-based functional analysis in mammalian stem cell differentiation*
**作者**：Madigan, S.L., et al.
**摘要**：研究利用哺乳动物细胞系，通过TRA-2α抗体阻断实验，发现TRA-2α通过调节TGF-β信号通路影响干细胞向生殖细胞的分化，为跨物种保守性机制提供了证据。
---
这些文献展示了TRA-2α抗体在基因调控、亚细胞定位及功能分析中的多样化应用。如需具体期刊信息或更多文献，可进一步限定研究领域或物种。
The TRA-2α antibody is a tool used to detect the Transforming Growth Factor Beta (TGF-β) Receptor-Associated Protein 2 Alpha (TRAP-2α or TRA-2α), a component of the TGF-β signaling pathway. TGF-β signaling regulates diverse cellular processes, including proliferation, differentiation, and apoptosis, and is implicated in development, immune regulation, and diseases like cancer and fibrosis. TRA-2α interacts with TGF-β receptors, acting as a co-receptor or adaptor to modulate Smad-dependent and non-Smad signaling cascades.
This antibody is commonly employed in research to study TRA-2α's expression, localization, and function in cellular and tissue contexts. It is used in techniques such as Western blotting, immunoprecipitation, and immunohistochemistry. TRA-2α is conserved across species, and its homologs are studied in model organisms (e.g., *C. elegans*) to explore developmental processes. In mammals, it plays roles in embryogenesis, stem cell regulation, and disease progression.
Studies using TRA-2α antibodies have highlighted its involvement in cancer metastasis, where TGF-β signaling often shifts from tumor-suppressive to pro-oncogenic. Dysregulation of TRA-2α has been linked to altered TGF-β responses in fibrotic diseases and immune evasion. The antibody’s specificity for TRA-2α, validated through knockdown/knockout controls, makes it critical for distinguishing its activity from related proteins like TRA-2β. Commercial TRA-2α antibodies are typically raised in rabbits or mice, targeting epitopes within conserved regions of the protein. Its applications span basic research and therapeutic target validation, particularly in contexts where TGF-β pathway dysregulation is pathological.
×
