| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CBLC |
| Uniprot No | Q9ULV8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-474aa |
| 氨基酸序列 | MALAVAPWGRQWEEARALGRAVRMLQRLEEQCVDPRLSVSPPSLRDLLPR TAQLLREVAHSRRAAGGGGPGGPGGSGDFLLIYLANLEAKSRQVAALLPP RGRRSANDELFRAGSRLRRQLAKLAIIFSHMHAELHALFPGGKYCGHMYQ LTKAPAHTFWRESCGARCVLPWAEFESLLGTCHPVEPGCTALALRTTIDL TCSGHVSIFEFDVFTRLFQPWPTLLKNWQLLAVNHPGYMAFLTYDEVQER LQACRDKPGSYIFRPSCTRLGQWAIGYVSSDGSILQTIPANKPLSQVLLE GQKDGFYLYPDGKTHNPDLTELGQAEPQQRIHVSEEQLQLYWAMDSTFEL CKICAESNKDVKIEPCGHLLCSCCLAAWQHSDSQTCPFCRCEIKGWEAVS IYQFHGQATAEDPGNSSDQEGRELELGQVPLSAPPLPPRPDLPPRKPRNA QPKVRLLKGNSPPAALGPQDPAPA |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CBLC重组蛋白的3篇文献参考(内容基于学术研究典型方向概括)：
1. **文献名称**：*Functional characterization of CBLC recombinant protein in EGFR degradation*
**作者**：Zhang L, et al.
**摘要**：研究利用大肠杆菌系统表达并纯化CBLC重组蛋白，验证其作为E3泛素连接酶的活性，证明其通过促进EGFR泛素化调控受体降解，为癌症靶向治疗提供依据。
2. **文献名称**：*Structural insights into the unique substrate specificity of CBLC in the CBL protein family*
**作者**：Wang Y, et al.
**摘要**：通过X射线晶体学解析CBLC重组蛋白结构，发现其区别于CBL和CBL-B的底物结合域特征，揭示其选择性调控特定信号通路的分子机制。
3. **文献名称**：*CBLC recombinant protein inhibits tumor growth by modulating immune signaling pathways*
**作者**：Garcia-Rios E, et al.
**摘要**：在哺乳动物细胞中表达CBLC重组蛋白，发现其通过增强T细胞活性及抑制STAT3信号通路抑制小鼠模型中黑色素瘤的生长，提示其免疫治疗潜力。
(注：以上为基于研究方向的模拟摘要，实际文献需通过PubMed或Google Scholar检索确认。)
**Background of CBLC Recombinant Protein**
CBLC (CBL Proto-Oncogene C) is a member of the CBL protein family, which includes evolutionarily conserved regulators (CBL, CBLB, and CBLC) involved in ubiquitination-mediated signaling modulation. These proteins act as E3 ubiquitin ligases, tagging target substrates with ubiquitin molecules to regulate their degradation or activity. CBLC shares structural homology with its family members, featuring a tyrosine kinase-binding (TKB) domain for phosphotyrosine recognition, a RING finger domain for catalytic ubiquitin transfer, and a proline-rich region for protein interactions.
Originally identified as an oncogene, CBLC plays dual roles in cellular signaling. It regulates receptor tyrosine kinase (RTK) pathways, such as EGFR and MET, by promoting ligand-dependent ubiquitination and endocytosis, thereby fine-tuning cell proliferation, differentiation, and survival. Unlike CBL and CBLB, which are broadly expressed, CBLC exhibits restricted tissue distribution, suggesting specialized functions in specific contexts, including immune regulation and cancer progression. Dysregulation of CBLC has been implicated in malignancies, immune disorders, and metabolic diseases, though its mechanisms remain less characterized compared to other CBL proteins.
Recombinant CBLC protein is engineered using expression systems (e.g., *E. coli*, mammalian cells*) to produce purified, bioactive forms for research. It serves as a critical tool for studying CBL family dynamics, ubiquitination cascades, and pathway cross-talk. Researchers utilize CBLC recombinant proteins to investigate its interactions with signaling molecules, its role in cancer metastasis, and its potential as a therapeutic target. Additionally, it aids in developing inhibitors or stabilizers to modulate RTK-driven pathologies.
Despite progress, CBLC's full biological scope and therapeutic relevance require further exploration, particularly its tissue-specific functions and crosstalk with other post-translational modifiers. Advances in recombinant protein technology continue to enhance the study of CBLC, bridging gaps in understanding its contribution to cellular homeostasis and disease.
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