| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | LAG1 longevity assurance; SP260; TMSG1; Tumor metastasis-suppressor gene 1 protein; |
| Entrez GeneID | 6670; |
| WB Predicted band size | 82kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from C-terminal of human SP3/4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于SP3/4抗体的3篇参考文献及其摘要概括：
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1. **文献名称**: *"SP3 and SP4: Multifaceted regulators of neuronal gene expression"*
**作者**: Suske G, et al.
**摘要**: 该综述总结了转录因子SP3和SP4在神经系统中调控基因表达的功能差异，指出SP3抗体常用于染色质免疫沉淀(ChIP)和Western blot，验证其与靶基因启动子区的结合能力，而SP4抗体被用于研究其在神经元发育中的特异性作用。
2. **文献名称**: *"Generation and characterization of monoclonal antibodies specific for human SP4 transcription factor"*
**作者**: Lee J, et al.
**摘要**: 研究团队开发了针对人源SP4蛋白的单克隆抗体，通过免疫荧光和免疫组化验证其特异性，并发现该抗体在检测脑组织SP4表达水平时比多克隆抗体更具灵敏度，支持其在神经退行性疾病研究中的应用。
3. **文献名称**: *"Differential roles of SP1 and SP3/4 in tumor progression through epigenetic regulation of oncogenes"*
**作者**: Zhang Y, et al.
**摘要**: 研究利用SP3/4抗体进行ChIP-seq分析，发现SP3和SP4在多种癌细胞中共同调控促癌基因(如MYC)的染色质开放状态，且SP4抗体特异性检测到其在侵袭性肿瘤中的高表达，提示其作为潜在治疗靶点。
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以上文献内容需结合具体研究目的筛选，建议通过PubMed或Google Scholar输入标题进一步查阅全文。
SP3/4 antibodies are a class of monoclonal antibodies initially developed to target specific epitopes associated with tumor-associated glycoproteins, particularly in cancer research. These antibodies gained prominence for their ability to recognize conserved epitopes on mucin-type glycoproteins, such as TAG-72 and MUC1. which are overexpressed in various adenocarcinomas, including pancreatic, colorectal, and ovarian cancers. The "SP" designation often refers to their development through hybridoma technology, where spleen-derived B cells (SP) from immunized mice are fused with myeloma cells to produce immortalized antibody-secreting cell lines.
SP3/4 antibodies are particularly noted for their utility in immunohistochemistry (IHC) for diagnostic and prognostic applications. For example, SP3 (specific for TAG-72) and SP4 (targeting MUC1) have been used to differentiate malignant from benign tissues, assess tumor margins, and study glycoprotein expression patterns in cancer progression. Their specificity for heavily glycosylated epitopes makes them valuable tools for investigating post-translational modifications linked to malignancy.
Recent studies have explored their role in targeted therapies, such as antibody-drug conjugates or radioimmunotherapy, though clinical translation remains limited. Challenges include heterogeneity in glycoprotein expression across tumors and normal tissues, which affects specificity. Despite this, SP3/4 antibodies remain foundational in cancer biomarker research, reflecting their historical and ongoing relevance in understanding tumor biology and developing diagnostic panels.
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