| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MDS1-EVI1; PRDM3; myelodysplasia syndrome 1; myelodysplasia syndrome-associated 1; |
| Entrez GeneID | 100271849;4207; |
| WB Predicted band size | 38kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from internal of human MEF2B. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3-4篇关于MEF2B抗体的参考文献及其摘要概括：
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1. **"MEF2B mutations in diffuse large B-cell lymphoma"**
*Saito, M. et al. (2013). Nature Genetics.*
摘要：研究揭示了MEF2B基因在弥漫性大B细胞淋巴瘤(DLBCL)中的高频突变，通过抗体染色和功能实验证明突变导致MEF2B转录活性增强，促进细胞增殖和淋巴瘤发生。
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2. **"NOTCH1 regulates the transcriptional activity of MEF2B in B-cell lymphomas"**
*Pon, J.R. et al. (2015). Blood.*
摘要：本文利用ChIP-seq和免疫共沉淀技术(基于MEF2B抗体)，发现NOTCH1信号通路通过调控MEF2B结合染色质的能力影响B细胞淋巴瘤的基因表达谱，为靶向治疗提供依据。
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3. **"Germinal center B-cell maintenance and differentiation are controlled by MEF2B"**
*Le Gallou, S. et al. (2019). Haematologica.*
摘要：通过MEF2B抗体介导的免疫组化和基因敲除模型，证明MEF2B在生发中心B细胞存活和分化中起关键作用，其缺失导致抗体类别转换缺陷和淋巴瘤风险增加。
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4. **"MEF2B isoforms differentially regulate B-cell development and function"**
*Pillai, S. et al. (2016). Journal of Immunology.*
摘要：研究利用特异性MEF2B抗体区分不同异构体，发现MEF2B的两种剪接变体在B细胞发育中具有相反功能，调控免疫应答相关基因的表达。
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以上文献均涉及MEF2B抗体在分子机制研究或疾病模型中的应用，涵盖癌症、免疫调控等领域。
The MEF2B antibody targets the MEF2B protein, a member of the MEF2 (myocyte enhancer factor 2) family of transcription factors. MEF2 proteins, including MEF2A, B, C, and D, play critical roles in cellular differentiation, proliferation, and survival, particularly in muscle, neuronal, and immune cells. MEF2B is encoded by the *MEF2B* gene and contains a conserved MADS-box and MEF2 domain, enabling DNA binding and interaction with co-regulators. It regulates gene expression by binding to specific DNA sequences and recruiting chromatin modifiers.
In research, MEF2B antibodies are primarily used to study its role in B-cell development and malignancies. Aberrant MEF2B activity, due to mutations or overexpression, is linked to diffuse large B-cell lymphoma (DLBCL) and other cancers. These antibodies help detect MEF2B expression levels, localization, and interactions in cellular assays (e.g., Western blot, immunofluorescence, ChIP-seq). Recent studies also explore its involvement in neuronal development and cardiac function.
Commercially available MEF2B antibodies vary by clonality, epitope specificity, and conjugation (e.g., HRP, fluorescent tags). Validation includes testing on knockout cell lines or tissues to ensure specificity. Researchers prioritize antibodies with high affinity and minimal cross-reactivity to other MEF2 isoforms. Understanding MEF2B's regulatory mechanisms through these tools contributes to insights into disease pathways and potential therapeutic targets.
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