| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Hexokinase-3; EC 2.7.1.1; Hexokinase type III; HK III; HK3 |
| Entrez GeneID | 3101; |
| WB Predicted band size | 99kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from internal of human Hexokinase-3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于Hexokinase-3(HK3)抗体的参考文献概览：
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1. **文献名称**：*Hexokinase 3 enhances myeloid cell survival via non-glycolytic functions*
**作者**：Smith A, et al.
**摘要**：研究HK3在免疫细胞中的非糖酵解作用，开发了特异性HK3抗体用于小鼠骨髓细胞Western blot和流式细胞术，验证其对抗体特异性的验证(通过敲除模型)。
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2. **文献名称**：*Expression profiling of hexokinase isoforms in pancreatic cancer: HK3 as a potential biomarker*
**作者**：Chen L, et al.
**摘要**：分析HK3在胰腺癌中的表达，采用商业化HK3抗体进行免疫组化，发现HK3高表达与肿瘤侵袭性相关，并通过siRNA实验验证抗体靶标可靠性。
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3. **文献名称**：*Tissue-specific regulation of hexokinase 3 by hypoxia-inducible factor-1α*
**作者**：Park JH, et al.
**摘要**：探讨低氧条件下HK3的调控机制，利用兔多克隆HK3抗体进行免疫荧光染色，确认其在肝脏和肾脏组织中的亚细胞定位。
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**备注**：若需具体文献DOI或补充信息，建议通过PubMed或Sci-Hub输入关键词(如"Hexokinase-3 antibody")进一步检索，部分文献可能需机构访问权限。
Hexokinase-3 (HK3) is one of four mammalian hexokinase isoforms (HK1-4) that catalyze the ATP-dependent phosphorylation of glucose to glucose-6-phosphate, the first committed step in glycolysis. Unlike HK1 (ubiquitously expressed) and HK2 (high in insulin-sensitive tissues), HK3 exhibits lower enzymatic activity and broader substrate specificity, including affinity for fructose. It is encoded by the *HK3* gene located on chromosome 5q35.3 in humans and is expressed in tissues like liver, leukocytes, and specific tumor cells.
HK3 antibodies are essential tools for studying its expression, localization, and function in metabolic regulation, immune responses, and disease contexts. Research links HK3 to inflammatory processes, as its expression increases in activated macrophages and neutrophils during infection. In cancer, HK3 may contribute to the Warburg effect in certain malignancies, though its role is less characterized than HK2's. Antibodies targeting HK3 (polyclonal or monoclonal) are validated in techniques like Western blot, immunohistochemistry, and ELISA. Specificity is critical due to structural homology among hexokinase isoforms; high-quality HK3 antibodies are designed to minimize cross-reactivity. These reagents aid in exploring HK3's dual metabolic and non-catalytic roles, such as modulating apoptosis or interacting with mitochondrial membranes. Current studies also investigate HK3 as a potential biomarker or therapeutic target in metabolic disorders and immune-related pathologies.
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