| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Diacylglycerol kinase epsilon; EC 2.7.1.107; Diglyceride kinase epsilon; DGK-epsilon; DAG kinase epsilon |
| Entrez GeneID | 8526; |
| WB Predicted band size | 60kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from internal of human DGKE. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与DGKE抗体或DGKE基因突变相关的代表性文献，摘要内容已概括：
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1. **文献名称**：*Mutations in DGKE cause atypical hemolytic-uremic syndrome*
**作者**：Lemaire M, et al.
**摘要**：该研究首次发现DGKE(diacylglycerol kinase ε)基因突变是非典型溶血尿毒症综合征(aHUS)的遗传因素之一。文章指出，DGKE突变导致细胞膜信号通路异常(如血管生成素通路)，进而引发内皮细胞损伤和血栓性微血管病，但未直接涉及自身抗体机制。
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2. **文献名称**：*DGKE variants in patients with complement-negative atypical HUS*
**作者**：Ozaltin F, et al.
**摘要**：本研究分析了补体阴性aHUS患者的基因特征，发现DGKE突变患者多表现为儿童期发病且存在复发性蛋白尿。文中提到，DGKE缺陷可能通过影响细胞因子信号(如Ras-MAPK通路)导致肾小球内皮损伤，但未明确讨论抗体的作用。
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3. **文献名称**：*Diacylglycerol kinase ε and the metabolic syndrome*
**作者**：Goto K, et al.
**摘要**：综述了DGKE在脂质代谢和胰岛素信号中的功能，提出DGKE异常可能导致代谢综合征和肾脏病变。文章间接提示DGKE可能成为自身免疫性疾病的潜在靶点，但未具体涉及抗体相关研究。
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**备注**：目前公开文献中，针对DGKE自身抗体的直接研究较少，上述文献主要聚焦于基因突变致病的机制。若需抗体相关研究，建议关注DGKE在肾小球疾病中的免疫组化应用或特定自身免疫模型中的探索。
DGKE (diacylglycerol kinase epsilon) is a lipid kinase that phosphorylates diacylglycerol (DAG) to produce phosphatidic acid (PA), playing a critical role in regulating cellular signaling pathways. As a member of the diacylglycerol kinase family, DGKE modulates the balance between DAG and PA, which are essential lipid second messengers involved in processes like membrane trafficking, cell proliferation, and immune responses. DGKE is uniquely characterized by its distinct structural domains, including a pleckstrin homology domain and a catalytic region, which enable its localization and activity at specific membrane compartments.
Mutations in the DGKE gene have been linked to kidney diseases, particularly atypical hemolytic uremic syndrome (aHUS), a rare genetic disorder characterized by thrombotic microangiopathy. Research suggests DGKE deficiency disrupts endothelial and platelet cell signaling, promoting prothrombotic and inflammatory states. DGKE antibodies are vital tools for studying these mechanisms, enabling the detection and quantification of DGKE protein expression in tissues and cells. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to explore DGKE’s role in health and disease. Recent studies also implicate DGKE in immune regulation and cancer, broadening its therapeutic research relevance. These antibodies help unravel DGKE’s complex interactions in lipid-mediated signaling, offering insights into potential targeted therapies for DGKE-associated pathologies.
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