| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM20B |
| Uniprot No | O75063 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 31-409aa |
| 氨基酸序列 | SAANREDQRA FHRMMTGLRV ELAPKLDHTL QSPWEIAAQW VVPREVYPEE TPELGAVMHA MATKKIIKAD VGYKGTQLKA LLILEGGQKV VFKPKRYSRD HVVEGEPYAG YDRHNAEVAA FHLDRILGFH RAPLVVGRFV NLRTEIKPVA TEQLLSTFLT VGNNTCFYGK CYYCRETEPA CADGDIMEGS VTLWLPDVWP LQKHRHPWGR TYREGKLARW EYDESYCDAV KKTSPYDSGP RLLDIIDTAV FDYLIGNADR HHYESFQDDE GASMLILLDN AKSFGNPSLD ERSILAPLYQ CCIIRVSTWN RLNYLKNGVL KSALKSAMAH DPISPVLSDP HLDAVDQRLL SVLATVKQCT DQFGMDTVLV EDRMPLSHL |
| 预测分子量 | 71 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FAM20B重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*FAM20B is a kinase that phosphorylates xylose in the glycosaminoglycan-protein linkage region*
**作者**：Koike T et al.
**摘要**：该研究揭示了FAM20B作为激酶，特异性磷酸化糖胺聚糖(GAG)与核心蛋白连接区的木糖残基，促进GAG链的延伸。通过重组蛋白实验证实其体外激酶活性，并表明该磷酸化对硫酸乙酰肝素/肝素的生物合成至关重要。
2. **文献名称**：*FAM20B regulates the synthesis of heparan sulfate/heparin in cultured cells*
**作者**：Wang S et al.
**摘要**：文章通过表达重组FAM20B蛋白，证明其调控肝素/硫酸乙酰肝素的生物合成。敲低FAM20B导致GAG链长度缩短，提示其在GAG链延伸中的直接作用，可能与木糖磷酸化介导的蛋白酶解调控相关。
3. **文献名称**：*The FAM20 family of secreted proteins: Structure, function, and disease associations*
**作者**：Tagliabracci VS et al.
**摘要**：综述中提及FAM20B作为分泌型激酶家族成员，解析其重组蛋白的结构特征，并讨论其通过磷酸化修饰调控细胞外基质成分的机制，以及与骨骼发育异常等疾病的潜在关联。
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以上研究均涉及FAM20B重组蛋白的功能验证，涵盖激酶活性、糖胺聚糖合成调控及病理意义等方面。
FAM20B, a member of the FAM20 (Family with sequence similarity 20) protein family, is a Golgi-associated kinase implicated in the regulation of glycosaminoglycan (GAG) biosynthesis. The FAM20 family comprises three members (FAM20A, FAM20B, and FAM20C), with FAM20B distinguished by its role in phosphorylating specific substrates involved in extracellular matrix (ECM) dynamics. Unlike FAM20C, a kinase for secreted phosphoproteins (e.g., SIBLING proteins), FAM20B catalyzes the phosphorylation of xylose residues on the tetrasaccharide linker of proteoglycans—a critical step in GAG chain initiation. This phosphorylation event ensures proper elongation and sulfation of GAGs, which are essential for ECM structure, cell signaling, and tissue homeostasis.
Recombinant FAM20B protein, typically produced in mammalian expression systems (e.g., HEK293 cells) to preserve post-translational modifications, serves as a tool to study its enzymatic activity, substrate specificity, and interaction networks. Its dysregulation has been linked to pathologies such as cancer, fibrosis, and skeletal disorders, where aberrant GAG synthesis disrupts ECM integrity or signaling pathways. For instance, FAM20B deficiency in models leads to impaired proteoglycan secretion and developmental defects, highlighting its physiological significance.
Research on recombinant FAM20B also explores its therapeutic potential. Inhibitors targeting FAM20B could modulate GAG-dependent processes in diseases like metastasis or chronic inflammation. Structural studies of the recombinant protein have revealed conserved kinase domains and regulatory motifs, aiding drug design. Despite progress, questions remain about its broader substrate spectrum and cross-talk with other FAM20 kinases. Overall, FAM20B recombinant protein bridges mechanistic insights into ECM biology and translational applications in ECM-related disorders.
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