| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/100-1/500 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CDC like kinase 1; CLK; CLK1; Dual specificity protein kinase CLK1; EC 2.7.1.112 |
| Entrez GeneID | 1195; |
| WB Predicted band size | 57kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from internal of human CLK1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于CLK1抗体的3篇参考文献示例(文献信息为虚构，仅供格式参考)：
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1. **"Characterization of CLK1-Specific Monoclonal Antibodies for Cancer Biomarker Studies"**
*作者：Bullock A.N., et al. (2019)*
**摘要**：本研究开发了一种高特异性的CLK1单克隆抗体，并通过Western blot和免疫组化验证其在结直肠癌组织中的表达。结果显示CLK1在肿瘤细胞核中高表达，与患者预后不良相关，提示其作为潜在生物标志物的价值。
2. **"CLK1 Inhibition Alters Splicing Factor Localization: Antibody-Based Validation in Cellular Models"**
*作者：Federov O., et al. (2017)*
**摘要**：利用CLK1抗体探究小分子抑制剂对RNA剪接因子亚细胞定位的影响。免疫荧光实验表明，抑制CLK1导致剪接因子SR蛋白从核斑点中解离，验证了CLK1在剪接调控中的关键作用。
3. **"Antibody-Driven Profiling of CLK1 Expression in Neurodegenerative Disorders"**
*作者：TzeplaevaL., et al. (2020)*
**摘要**：通过CLK1抗体分析阿尔茨海默病模型小鼠脑组织，发现CLK1蛋白水平在病变神经元中显著升高，并与tau蛋白异常磷酸化相关，提示其可能参与神经退行性病理机制。
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*注：以上文献为示例性质，实际研究中请通过PubMed或专业数据库检索真实文献。*
**Background of CLK1 Antibody**
The CDC-like kinase 1 (CLK1) is a serine/threonine kinase belonging to the CLK family, which regulates pre-mRNA splicing by phosphorylating serine/arginine-rich (SR) proteins. These modifications influence SR protein localization and interaction with spliceosomes, thereby modulating alternative splicing events critical for cellular processes like cell cycle progression, differentiation, and stress responses. CLK1’s role extends to various diseases, including cancer, neurodegenerative disorders, and viral infections, where dysregulated splicing contributes to pathogenesis.
CLK1 antibodies are essential tools for studying its expression, activity, and functional mechanisms. They enable detection of CLK1 in techniques like Western blotting, immunofluorescence, and immunohistochemistry, aiding in the exploration of its tissue-specific distribution and subcellular localization. Additionally, these antibodies are used in co-immunoprecipitation assays to identify CLK1-interacting proteins or assess its phosphorylation status. In drug discovery, CLK1 antibodies help evaluate the efficacy of kinase inhibitors targeting CLK1. which is emerging as a therapeutic target due to its involvement in splicing-related pathologies. Recent studies highlight CLK1’s potential in cancer therapy, as its inhibition can alter splicing patterns linked to tumor progression.
Overall, CLK1 antibodies are pivotal in unraveling the kinase’s biological roles and translational applications, bridging basic research and clinical innovation.
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