| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Homeobox protein Hox-D12; Hox-4H; HOXD12; HOX4H; |
| Entrez GeneID | 3238; |
| WB Predicted band size | 28kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from internal of human HOXD12. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于HOXD12抗体的示例参考文献(注：以下为模拟示例，具体文献需通过学术数据库检索确认)：
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1. **文献名称**: *HOXD12 Expression in Colorectal Cancer: Prognostic Implications*
**作者**: Zhang Y, et al.
**摘要**: 本研究通过HOXD12抗体进行免疫组化分析，发现HOXD12蛋白在结肠癌组织中高表达，且与患者预后不良及淋巴结转移显著相关。
2. **文献名称**: *HOXD12 Regulates Limb Development via Wnt Signaling*
**作者**: Smith J, et al.
**摘要**: 利用HOXD12抗体在小鼠胚胎中定位蛋白表达，揭示其在肢体形态发生中的作用，并证明其通过调控Wnt通路影响细胞分化。
3. **文献名称**: *HOXD12 as a Biomarker in Neuroblastoma Progression*
**作者**: Lee S, et al.
**摘要**: 通过Western blot和免疫荧光技术(基于HOXD12抗体)，发现HOXD12蛋白水平与神经母细胞瘤侵袭性呈正相关，提示其作为潜在治疗靶点。
4. **文献名称**: *HOXD12 in Epidermal Differentiation*
**作者**: Garcia R, et al.
**摘要**: 使用HOXD12抗体进行皮肤组织染色，证明其在表皮分层中的动态表达模式，并参与调控角质形成细胞分化。
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建议通过PubMed、Google Scholar等平台，以“HOXD12 antibody”及关键词(如“expression”“cancer”“development”)检索最新文献。
The HOXD12 antibody is a tool used to detect the HOXD12 protein, a member of the homeobox (HOX) gene family. HOX genes encode transcription factors critical for embryonic development, particularly in establishing body axis patterning, organogenesis, and limb formation. The HOXD12 gene, located on chromosome 2 (2q31.1 in humans), is part of the HOXD cluster and plays roles in posterior limb development and digit specification. Dysregulation of HOXD12 has been implicated in cancers, including colorectal, breast, and prostate malignancies, where it may influence tumor progression, metastasis, or drug resistance.
HOXD12 antibodies are typically developed in hosts like rabbits or mice using immunogenic peptides or recombinant protein fragments. These antibodies are validated for applications such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). Researchers utilize them to study HOXD12's expression patterns, regulatory mechanisms, and interactions with downstream targets in developmental and pathological contexts.
Commercial HOXD12 antibodies often undergo specificity testing via knockout cell lines or siRNA knockdown to ensure minimal cross-reactivity with other HOX proteins. Their utility extends to both basic research—exploring HOX gene networks—and clinical studies, such as biomarker discovery or therapeutic targeting in oncology. Proper validation and optimization for each experimental setup remain essential due to potential variability in antibody performance.
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