| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/100-1/500 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CF-1 64 kDa; CSTF 64 kDa; CstF-64; cleavage stimulation factor subunit 2; cleavage stimulation factor |
| Entrez GeneID | 1478; |
| WB Predicted band size | 60kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from N-terminal of human CSTF2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于CSTF2抗体的3篇示例参考文献(注：文献为模拟示例，建议通过学术数据库核实具体研究)：
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1. **文献名称**：*"A monoclonal antibody targeting CSTF2 disrupts mRNA 3' processing and inhibits tumor growth"*
**作者**：Zhang et al.
**摘要内容**：本研究开发了一种针对CSTF2的单克隆抗体，通过阻断其与RNA聚合酶II的相互作用，抑制mRNA的3'末端加工，从而减缓体外癌细胞增殖和小鼠模型中的肿瘤生长。
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2. **文献名称**：*"CSTF2 antibody-based profiling reveals its role in alternative polyadenylation during T cell activation"*
**作者**：Lee et al.
**摘要内容**：利用CSTF2特异性抗体进行免疫沉淀和RNA测序，揭示了CSTF2在T细胞激活过程中通过调控选择性多聚腺苷酸化(APA)影响免疫相关基因表达的机制。
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3. **文献名称**：*"Validation of a commercial CSTF2 antibody for diagnostic applications in myelodysplastic syndromes"*
**作者**：Garcia-Rodriguez et al.
**摘要内容**：验证了一种商品化CSTF2抗体的灵敏度和特异性，发现其在骨髓增生异常综合征(MDS)患者样本中能有效检测CSTF2的异常表达，为疾病诊断提供潜在标志物。
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**建议**：如需具体文献，可通过PubMed或Google Scholar检索关键词“CSTF2 antibody”或结合研究主题(如“CSTF2 cancer”或“CSTF2 mRNA processing”)筛选相关论文。
The CSTF2 antibody is a tool used to detect the Cleavage Stimulation Factor Subunit 2 (CSTF2), a critical component of the mRNA 3ʹ-end processing machinery. CSTF2. part of the CSTF complex (CSTF1. CSTF2. CSTF3), binds to GU-rich sequences downstream of mRNA polyadenylation signals, facilitating cleavage and poly-A tail addition during transcription termination. This process is essential for mRNA maturation, nuclear export, and stability. Dysregulation of CSTF2 has been linked to cancers, neurodegenerative disorders, and developmental defects, making it a research focus in gene expression studies.
CSTF2 antibodies are widely employed in techniques like Western blotting, immunoprecipitation, and immunofluorescence to analyze protein expression, localization, and interactions. They help investigate CSTF2’s role in diseases, such as its overexpression in certain tumors or reduced levels in neurological conditions. Commercial CSTF2 antibodies are typically raised in rabbits or mice, targeting specific epitopes (e.g., human CSTF2 residues 500-600). Validation includes testing for specificity using knockout cells or siRNA-mediated knockdown. Researchers also use these antibodies to study transcriptional dysregulation, alternative polyadenylation events, and potential therapeutic targets. Proper controls are crucial due to possible cross-reactivity with homologous proteins.
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