Rabbit Polyclonal CIITA Antibody

The CIITA (Class II Major Histocompatibility Complex Transactivator) antibody is a crucial tool for studying the regulation of MHC class II molecules, which play a central role in adaptive immunity by presenting antigens to CD4+ T cells. CIITA, a master transcriptional coactivator, controls MHC-II gene expression by recruiting chromatin modifiers and binding to the promoter region. Mutations in the CIITA gene cause type II bare lymphocyte syndrome, a severe immunodeficiency disorder. Researchers use CIITA antibodies to investigate its expression, localization, and interaction partners in immune cells like B cells, dendritic cells, and macrophages. These antibodies are applied in techniques such as Western blotting, immunofluorescence, and ChIP-seq to explore CIITA's role in autoimmune diseases (e.g., rheumatoid arthritis), viral immune evasion, and cancer immunology. Different isoforms (I-IV) generated by alternative promoters allow cell type-specific regulation, which can be distinguished using isoform-specific antibodies. Recent studies also examine CIITA's non-canonical functions in autophagy and cytokine signaling. Commercial CIITA antibodies are typically developed in hosts like rabbits or mice, with validation in knockout cell lines to ensure specificity.