| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/100-1/500 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | E74-like factor 1; ELF1; ETS-related transcription factor Elf-1; |
| Entrez GeneID | 1997; |
| WB Predicted band size | 67kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from C-terminal of human ELF1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于ELF1抗体的参考文献(均为虚构示例，仅用于演示格式)：
1. **文献名称**: "ELF1 transcription factor regulates T-cell receptor signaling through direct DNA binding"
**作者**: Smith A, et al.
**摘要**: 研究利用ELF1特异性抗体进行ChIP-seq分析，揭示了ELF1在T细胞受体信号通路中通过结合靶基因启动子调控免疫应答的分子机制。
2. **文献名称**: "Antibody-based detection of ELF1 in B-cell malignancies"
**作者**: Johnson R, et al.
**摘要**: 通过Western blot和免疫组化验证ELF1抗体的特异性，证明ELF1在B细胞淋巴瘤中异常高表达，并与患者预后显著相关。
3. **文献名称**: "ELF1 mediates interferon-γ response in macrophages via chromatin remodeling"
**作者**: Lee H, et al.
**摘要**: 使用ELF1抗体进行染色质免疫共沉淀(ChIP)，发现ELF1通过重塑巨噬细胞染色质结构调控干扰素-γ诱导的炎症相关基因表达。
注：以上文献为模拟内容，实际引用需通过PubMed/Google Scholar检索关键词"ELF1 antibody"+"应用方向"(如ChIP/WB/癌症类型)获取真实文献。
The ELF1 antibody is a crucial tool in studying the ETS family transcription factor ELF1 (E74-like factor 1), which plays pivotal roles in regulating gene expression during development, immunity, and oncogenesis. ELF1 binds to DNA through its conserved ETS domain, influencing cellular processes like proliferation, differentiation, and apoptosis. It is particularly significant in immune regulation, where it modulates T-cell and B-cell development, cytokine production, and response to pathogens. Dysregulation of ELF1 has been linked to autoimmune diseases and cancers, including leukemia and breast cancer, where it may act as either an oncogene or tumor suppressor depending on context.
The ELF1 antibody, typically raised in rabbits or mice using recombinant ELF1 protein or synthetic peptides, enables detection and analysis of ELF1 expression and function in research. It is widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). Specificity and validation are critical, as cross-reactivity with other ETS family members (e.g., ELK1. GABPA) can occur. Commercial antibodies often cite applications in studying transcriptional networks, immune cell signaling, and cancer pathways. Key studies using ELF1 antibodies have elucidated its role in IFN-γ signaling, viral response, and hematopoiesis, with foundational work dating to the 1990s. Recent research focuses on its therapeutic potential in targeting ELF1-driven malignancies or autoimmune disorders.
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