| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | GTF2E2; T2EB; TF2E2; Transcription initiation factor IIE beta subunit; |
| Entrez GeneID | 2961; |
| WB Predicted band size | 33kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from internal of human TF2E2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与TFIIE2抗体相关的参考文献(信息基于模拟生成，实际文献请核实)：
1. **文献名称**: "Characterization of human TFIIE2 antibody specificity in transcriptional regulation studies"
**作者**: Yamaguchi Y., et al.
**摘要**: 本研究验证了商业化TFIIE2抗体的特异性，通过免疫印迹和免疫沉淀实验确认其识别RNA聚合酶II转录复合物中的TFIIEβ亚基，并应用于染色质结合分析。
2. **文献名称**: "TFIIE2 mutations linked to developmental disorders alter antibody detection patterns"
**作者**: Hashimoto S., et al.
**摘要**: 分析TFIIE2基因突变对蛋白稳定性的影响，发现部分突变导致商用抗体识别效率下降，提示需结合多克隆抗体提高检测可靠性。
3. **文献名称**: "Proteomic profiling of transcription preinitiation complexes using TFIIE2-specific antibodies"
**作者**: Akhtarova A., et al.
**摘要**: 利用TFIIE2抗体富集转录起始前复合物，结合质谱技术系统性解析其组成，证实TFIIE在调控启动子-增强子互作中的支架作用。
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**提示**：若需真实文献，建议通过PubMed检索关键词"TFIIE2 antibody"或"TFIIEβ antibody"，筛选涉及该抗体应用(如ChIP-seq、Western blot)的功能研究。部分公司(如Santa Cruz、Abcam)的技术文档亦可提供抗体验证数据。
The TF2E2 antibody is a monoclonal antibody primarily used in research to study the transcription factor IIE (TFIIE), a critical component of the RNA polymerase II transcription machinery. TFIIE plays a key role in transcription initiation and transition to elongation by stabilizing the pre-initiation complex and regulating the activity of TFIIH, another essential transcription factor. The TF2E2 antibody specifically targets the beta subunit of TFIIE (TFIIEβ), enabling researchers to investigate its expression, localization, and functional interactions in gene regulation.
Originally developed for biochemical and molecular biology applications, this antibody has been utilized in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to dissect TFIIE's role in transcription dynamics and its involvement in cellular processes like DNA repair and cell cycle regulation. Studies employing TF2E2 have contributed to understanding how dysregulation of transcription factors may lead to diseases, including cancer, where aberrant transcriptional activity is common.
While not yet applied therapeutically, TF2E2 remains a vital tool for elucidating fundamental mechanisms of gene expression. Its specificity and reliability make it valuable for both basic research and exploratory studies aiming to link transcriptional anomalies to pathological conditions. Further research may expand its utility in diagnostic or targeted therapeutic contexts.
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