| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SRPK3 |
| Uniprot No | Q9UPE1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-567aa |
| 氨基酸序列 | MSASTGGGGD SGGSGGSSSS SQASCGPESS GSELALATPV PQMLQGLLGS DDEEQEDPKD YCKGGYHPVK IGDVFNGRYH VVRKLGWGHF STVWLCWDIQ RKRFVALKVV KSAGHYTETA VDEIKLLKCV RDSDPSDPKR ETIVQLIDDF RISGVNGVHV CMVLEVLGHQ LLKWIIKSNY QGLPVPCVKS IVRQVLHGLD YLHTKCKIIH TDIKPENILL CVGDAYIRRL AAEATEWQQA GAPPPSRSIV STAPQEVLQT GKLSKNKRKK MRRKRKQQKR LLEERLRDLQ RLEAMEAATQ AEDSGLRLDG GSGSTSSSGC HPGGARAGPS PASSSPAPGG GRSLSAGSQT SGFSGSLFSP ASCSILSGSS NQRETGGLLS PSTPFGASNL LVNPLEPQNA DKIKIKIADL GNACWVHKHF TEDIQTRQYR AVEVLIGAEY GPPADIWSTA CMAFELATGD YLFEPHSGED YSRDEDHIAH IVELLGDIPP AFALSGRYSR EFFNRRGELR HIHNLKHWGL YEVLMEKYEW PLEQATQFSA FLLPMMEYIP EKRASAADCL QHPWLNP |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SRPK3重组蛋白的3篇参考文献及其摘要概括：
1. **标题**: "SRPK3. a novel splicing factor kinase involved in myotonic dystrophy"
**作者**: Wang et al.
**摘要**: 该研究首次克隆并表达了SRPK3重组蛋白，发现其在骨骼肌中高表达。通过体外激酶实验证实其对SR蛋白的磷酸化能力，并揭示其异常活性与强直性肌营养不良中剪接失调相关。
2. **标题**: "Recombinant SRPK3 characterization and its role in muscle development"
**作者**: Nakamura et al.
**摘要**: 报道了SRPK3重组蛋白在大肠杆菌中的高效表达与纯化方法。功能研究表明，SRPK3通过调控mRNA剪接参与肌管分化，敲低后导致小鼠模型出现肌肉萎缩表型。
3. **标题**: "Structural insights into SRPK3 activation mechanism"
**作者**: Chen et al.
**摘要**: 解析了SRPK3重组蛋白的晶体结构，揭示其独特的激酶结构域构象。通过突变实验验证了ATP结合口袋的关键氨基酸，为开发选择性抑制剂治疗肌肉疾病提供了结构基础。
注：上述文献信息为示例性概括，实际文献需通过数据库检索确认。建议使用PubMed/Google Scholar以“SRPK3 recombinant”、“SRPK3 kinase activity”为关键词查找最新研究。
SRPK3 (Serine-Arginine Protein Kinase 3) is a member of the SRPK family, which regulates pre-mRNA splicing by phosphorylating serine-arginine (SR) repeat domains in splicing factors. Unlike its widely expressed homologs SRPK1 and SRPK2. SRPK3 exhibits tissue-specific expression, predominantly in skeletal muscle and cardiac tissue. This kinase shares a conserved N-terminal kinase domain but diverges in its C-terminal regulatory regions, influencing substrate specificity and subcellular localization.
SRPK3 gained attention due to its role in muscle development and disease. Studies link it to myogenic differentiation, where it modulates the activity of splicing factors like SRSF1. impacting alternative splicing of muscle-specific transcripts. Notably, SRPK3 knockout mice display muscular atrophy and impaired regeneration, highlighting its importance in muscle homeostasis. Mutations or dysregulation of SRPK3 have been associated with human myopathies, including Duchenne muscular dystrophy (DMD), where aberrant splicing exacerbates pathology.
Recombinant SRPK3 proteins are engineered using bacterial or mammalian expression systems, often fused with tags (e.g., GST, His) for purification and functional studies. These proteins enable in vitro kinase assays, structural analysis (e.g., crystallography), and screening for inhibitors targeting SRPK3-driven pathways. Current research explores SRPK3’s dual role as a splicing regulator and potential therapeutic target, particularly in muscle-wasting disorders and cancers with splicing dysregulation. Its tissue-restricted expression makes it an attractive candidate for selective intervention, though mechanistic details remain under investigation.
×
