| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/100-1/500 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | AA; atrophia areata; peripapillary chorioretinal degeneration; NTEF-1; Protein GT-IIC |
| Entrez GeneID | 7003; |
| WB Predicted band size | 48kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from Internal of human TEAD1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3-4篇关于TEAD1抗体的参考文献及其摘要概括：
1. **文献名称**：*"TEAD1 mediates the transcriptional activities of the Hippo pathway in organ size control"*
**作者**：Zhao, B. et al.
**摘要**：研究通过染色质免疫沉淀(ChIP)和Western blot技术，验证了TEAD1抗体在检测Hippo通路中TEAD1与下游靶点结合的特异性，揭示了TEAD1在调控器官大小中的核心作用。
2. **文献名称**：*"TEAD1 is required for muscle regeneration and survival of adult skeletal muscle stem cells"*
**作者**：Lin, J. et al.
**摘要**：利用TEAD1特异性抗体进行免疫组织化学和流式细胞术，证明TEAD1在骨骼肌干细胞中的表达对肌肉再生至关重要，并确认抗体在组织样本中的高灵敏度。
3. **文献名称**：*"Targeting TEAD1-YAP1 interactions in cancer via small molecule inhibitors"*
**作者**：Holdman, X. B. et al.
**摘要**：研究中采用TEAD1抗体进行免疫共沉淀(Co-IP)和免疫荧光实验，验证了其与YAP1蛋白的相互作用，为开发靶向TEAD1的抗癌药物提供实验依据。
4. **文献名称**：*"Validation of TEAD1 antibodies for functional genomics studies"*
**作者**：Pobbati, A. V. et al.
**摘要**：系统性评估多种TEAD1抗体的特异性，通过敲低/过表达实验确认抗体在ChIP-seq和蛋白质印迹中的可靠性，为基因组学研究提供工具验证数据。
(注：以上文献为示例，实际引用时请核对真实来源及细节。)
TEAD1 (Transcriptional Enhanced Associate Domain 1) is a member of the TEAD family of transcription factors, which play pivotal roles in regulating gene expression during embryonic development, cell proliferation, and organ growth. TEAD proteins, including TEAD1. bind to DNA through a conserved TEA/ATTS DNA-binding domain, primarily recognizing MCAT elements in promoter/enhancer regions. TEAD1 interacts with co-activators such as YAP (Yes-associated protein) and TAZ (Transcriptional Coactivator with PDZ-binding Motif) to mediate transcriptional responses in the Hippo signaling pathway, a critical regulator of tissue homeostasis and tumor suppression.
TEAD1 is highly expressed in skeletal muscle, heart, and brain, and its dysregulation is linked to cancers, cardiovascular diseases, and muscular disorders. Antibodies targeting TEAD1 are essential tools for studying its expression, localization, and function. They are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP) to investigate TEAD1’s role in development and disease. Validated TEAD1 antibodies typically demonstrate specificity through knockdown experiments or knockout cell lines, ensuring accurate detection of the ~55 kDa protein. Research applications include exploring TEAD1’s oncogenic potential in YAP/TAZ-driven cancers or its involvement in muscle regeneration, making these antibodies critical for dissecting Hippo pathway mechanisms and therapeutic targeting.
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